論文 - 詳細
| RRC ID | 3525 |
|---|---|
| 著者 | Nelms BL, Hanna-Rose W. |
| タイトル | C. elegans HIM-8 functions outside of meiosis to antagonize EGL-13 Sox protein function. |
| ジャーナル | Dev Biol |
| Abstract |
egl-13 encodes a Sox domain protein that is required for proper uterine seam cell development in Caenorhabditis elegans. We demonstrate that mutations of the C2H2 zinc fingers encoded by the him-8 (high incidence of males) gene partially suppress the egg-laying and connection-of-gonad morphology defects caused by incompletely penetrant alleles of egl-13. him-8 alleles have previously characterized recessive effects on recombination and segregation of the X chromosome during meiosis due to failure of X chromosome homolog pairing and subsequent synapsis. However, we show that him-8 alleles are semi-dominant suppressors of egl-13, and the semi-dominant effect is due to haplo-insufficiency of the him-8 locus. Thus, we conclude that the wild-type him-8 gene product acts antagonistically to EGL-13. Null alleles of egl-13 cannot be suppressed, suggesting that this antagonistic interaction most likely occurs either upstream of or in parallel with EGL-13. Moreover, we conclude that suppression of egl-13 is due to a meiosis-independent function of him-8 because suppression is observed in mutants that have severely reduced meiotic germ cell populations and suppression does not depend on the function of him-8 in the maternal germ line. We also show that the chromosomal context of egl-13 seems important in the him-8 suppression mechanism. Interactions between these genes can give insight into function of Sox family members, which are important in many aspects of metazoan development, and into functions of him-8 outside of meiosis. |
| 巻・号 | 293(2) |
| ページ | 392-402 |
| 公開日 | 2006-5-15 |
| DOI | 10.1016/j.ydbio.2006.02.010 |
| PII | S0012-1606(06)00104-7 |
| PMID | 16546157 |
| MeSH | Alleles Animals Animals, Genetically Modified Base Sequence Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / antagonists & inhibitors* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* DNA, Helminth / genetics Female Genes, Helminth Male Meiosis Mutation RNA Interference Suppression, Genetic Transcription Factors / antagonists & inhibitors* Transcription Factors / genetics Transcription Factors / metabolism Uterus / growth & development Vulva / growth & development |
| IF | 2.896 |
| 引用数 | 5 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm611 |