RRC ID 3526
Author Nomura H, Athauda SB, Wada H, Maruyama Y, Takahashi K, Inoue H.
Title Identification and reverse genetic analysis of mitochondrial processing peptidase and the core protein of the cytochrome bc1 complex of Caenorhabditis elegans, a model parasitic nematode.
Journal J. Biochem.
Abstract Mitochondria could be a good target for anti-parasitic drugs. The alpha and beta subunits of mitochondrial processing peptidase (MPP) and the core subunits of the cytochrome bc1 complex, UCR-1 and UCR-2, are homologous to one another and are important for mitochondrial functions. However, our knowledge of these proteins in nematodes is very limited. Caenorhabditis elegans, a free-living nematode, has six genes coding for proteins homologous to these subunits. On primary structure comparison, and immunochemical and enzymological analyses, the gene products were assigned as follows: Y71G12B.24, alpha-MPP; ZC410.2, beta-MPP; F56D2.1, UCR-1; VW06B3R.1, T10B10.2; and T24C4.1, UCR-2. The primary structures of beta-MPP and UCR-1 from Brugia malayi, a parasitic nematode causing human filariasis, were deduced from their cDNA structures. Phylogenetic analysis showed that the UCR-1s from both C. elegans and B. malayi were less related to mammalian UCR-1s than to MPPs from various organisms. MPP and the bc1 complex are essential for the life cycle of C. elegans, because their reverse genetic inhibition is lethal. This suggests the possibility that these proteins are also essential for the viability of B. malayi and other parasitic nematodes, and are potential targets for anti-parasitic agents.
Volume 139(6)
Pages 967-79
Published 2006-6
DOI 10.1093/jb/mvj114
PII 139/6/967
PMID 16788047
MeSH Amino Acid Motifs / genetics Amino Acid Sequence Animals Blotting, Western Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism DNA, Complementary / genetics Electron Transport Complex III / chemistry Electron Transport Complex III / genetics* Electron Transport Complex III / metabolism Humans Metalloendopeptidases / chemistry Metalloendopeptidases / genetics* Metalloendopeptidases / metabolism Mitochondrial Proteins / chemistry Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism Molecular Sequence Data Mutagenesis, Site-Directed Nematoda / genetics Nematoda / metabolism Phylogeny Protein Structure, Quaternary RNA Interference Recombinant Proteins / chemistry Recombinant Proteins / metabolism Sequence Homology, Amino Acid Substrate Specificity
IF 2.35
Times Cited 6
C.elegans tm1181 tm1326