RRC ID 35261
著者 Gold VA, Robson A, Bao H, Romantsov T, Duong F, Collinson I.
タイトル The action of cardiolipin on the bacterial translocon.
ジャーナル Proc Natl Acad Sci U S A
Abstract Cardiolipin is an ever-present component of the energy-conserving inner membranes of bacteria and mitochondria. Its modulation of the structure and dynamism of the bilayer impacts on the activity of their resident proteins, as a number of studies have shown. Here we analyze the consequences cardiolipin has on the conformation, activity, and localization of the protein translocation machinery. Cardiolipin tightly associates with the SecYEG protein channel complex, whereupon it stabilizes the dimer, creates a high-affinity binding surface for the SecA ATPase, and stimulates ATP hydrolysis. In addition to the effects on the structure and function, the subcellular distribution of the complex is modified by the cardiolipin content of the membrane. Together, the results provide rare and comprehensive insights into the action of a phospholipid on an essential transport complex, which appears to be relevant to a broad range of energy-dependent reactions occurring at membranes.
巻・号 107(22)
ページ 10044-9
公開日 2010-6-1
DOI 10.1073/pnas.0914680107
PII 0914680107
PMID 20479269
PMC PMC2890433
MeSH Adenosine Triphosphatases / chemistry Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism Adenosine Triphosphate / metabolism Apraxia, Ideomotor Bacterial Proteins / chemistry Bacterial Proteins / genetics Bacterial Proteins / metabolism Binding Sites Cardiolipins / metabolism Cardiolipins / pharmacology* Escherichia coli / drug effects* Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / chemistry Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Fluorescent Dyes Membrane Transport Proteins / chemistry Membrane Transport Proteins / genetics Membrane Transport Proteins / metabolism Models, Molecular Multiprotein Complexes Protein Stability / drug effects Protein Transport / drug effects* SEC Translocation Channels SecA Proteins
IF 9.412
引用数 90
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌)