| RRC ID |
35271
|
| 著者 |
Kapoor S, Das G, Varshney U.
|
| タイトル |
Crucial contribution of the multiple copies of the initiator tRNA genes in the fidelity of tRNA(fMet) selection on the ribosomal P-site in Escherichia coli.
|
| ジャーナル |
Nucleic Acids Res
|
| Abstract |
The accuracy of the initiator tRNA (tRNA(fMet)) selection in the ribosomal P-site is central to the fidelity of protein synthesis. A highly conserved occurrence of three consecutive G-C base pairs in the anticodon stem of tRNA(fMet) contributes to its preferential selection in the P-site. In a genetic screen, using a plasmid borne copy of an inactive tRNA(fMet) mutant wherein the three G-C base pairs were changed, we isolated Escherichia coli strains that allow efficient initiation with the tRNA(fMet) mutant. Here, extensive characterization of two such strains revealed novel mutations in the metZWV promoter severely compromising tRNA(fMet) levels. Low cellular abundance of the chromosomally encoded tRNA(fMet) allows efficient initiation with the tRNA(fMet) mutant and an elongator tRNA(Gln), revealing that a high abundance of the cellular tRNA(fMet) is crucial for the fidelity of initiator tRNA selection on the ribosomal P-site in E. coli. We discuss possible implications of the changes in the cellular tRNA(fMet) abundance in proteome remodeling.
|
| 巻・号 |
39(1)
|
| ページ |
202-12
|
| 公開日 |
2011-1-1
|
| DOI |
10.1093/nar/gkq760
|
| PII |
gkq760
|
| PMID |
20798174
|
| PMC |
PMC3017606
|
| MeSH |
Anticodon / chemistry
Base Pairing
Chromosome Mapping
Cold Temperature
Escherichia coli / genetics*
Escherichia coli / growth & development
Escherichia coli / metabolism
Genetic Loci
Mutation
Peptide Chain Initiation, Translational*
RNA, Transfer, Gln / metabolism
RNA, Transfer, Met / chemistry
RNA, Transfer, Met / genetics*
RNA, Transfer, Met / metabolism
Ribosomes / chemistry
Ribosomes / metabolism*
Suppression, Genetic
|
| IF |
11.502
|
| 引用数 |
20
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
|
