RRC ID 35284
Author Shoji S, Janssen BD, Hayes CS, Fredrick K.
Title Translation factor LepA contributes to tellurite resistance in Escherichia coli but plays no apparent role in the fidelity of protein synthesis.
Journal Biochimie
Abstract LepA is a translational GTPase highly conserved in bacterial lineages. While it has been shown that LepA can catalyze reverse ribosomal translocation in vitro, the role of LepA in the cell remains unclear. Here, we show that deletion of the lepA gene (DeltalepA) in Escherichia coli causes hypersensitivity to potassium tellurite and penicillin G, but has no appreciable effect on growth under many other conditions. DeltalepA does not increase miscoding or frameshifting errors under normal or stress conditions, indicating that LepA does not contribute to the fidelity of translation. Overexpression of LepA interferes with tmRNA-mediated peptide tagging and A-site mRNA cleavage, suggesting that LepA is a bona fide translation factor that can act on stalled ribosomes with a vacant A site in vivo. Together these results lead us to hypothesize that LepA is involved in co-translational folding of proteins that are otherwise vulnerable to tellurite oxidation.
Volume 92(2)
Pages 157-63
Published 2010-2-1
DOI 10.1016/j.biochi.2009.11.002
PII S0300-9084(09)00306-X
PMID 19925844
PMC PMC2815024
MeSH Drug Resistance, Bacterial* Escherichia coli / cytology Escherichia coli / drug effects* Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / biosynthesis* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism GTP Phosphohydrolases / deficiency GTP Phosphohydrolases / genetics Gene Deletion Oxidants / pharmacology Peptide Initiation Factors Phosphoproteins / deficiency Phosphoproteins / genetics Protein Biosynthesis / physiology* RNA, Messenger / genetics Tellurium / pharmacology* Transcriptional Elongation Factors / deficiency Transcriptional Elongation Factors / genetics Transcriptional Elongation Factors / metabolism*
IF 3.413
Times Cited 40
Prokaryotes E. coli ME9062(BW25113)(Keio) JW2553-KC JW5771-KC