| RRC ID |
35317
|
| Author |
Tashiro Y, Fukutomi H, Terakubo K, Saito K, Umeno D.
|
| Title |
A nucleoside kinase as a dual selector for genetic switches and circuits.
|
| Journal |
Nucleic Acids Res
|
| Abstract |
The development of genetic switches and their integrated forms (genetic circuits) with desired specifications/functions is key for success in synthetic biology. Due to the difficulty in rational design, genetic switches and circuits with desirable specifications are mostly obtained by directed evolution. Based on a virus-derived nucleotide kinase as a single-gene dual selector, we constructed a robust, efficient and stringent selection system for genetic switches. This method exhibited unprecedented enrichment efficacy (>30,000-fold) of functional switches from non-functional ones in a single selection cycle. In addition, negative (OFF) selection was exceptionally stringent, allowing the rapid and efficient selection of non-leaky from leaky circuits.
|
| Volume |
39(3)
|
| Pages |
e12
|
| Published |
2011-2-1
|
| DOI |
10.1093/nar/gkq1070
|
| PII |
gkq1070
|
| PMID |
21062820
|
| PMC |
PMC3035434
|
| MeSH |
Gene Regulatory Networks*
Genetic Engineering / methods*
Models, Genetic
Simplexvirus / enzymology
Thymidine Kinase / metabolism*
|
| IF |
11.502
|
| Times Cited |
23
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Prokaryotes E. coli |
JW1226-KC |
