論文 - 詳細
| RRC ID | 35382 |
|---|---|
| 著者 | Miyoshi K, Matsuura H, Kumon T, Matsuda K, Yoshikawa T, Bamba T, Harada K, Hirata K. |
| タイトル | Isolation and partial characterization of the Chlamydomonas reinhardtii γ-glutamyl kinase. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
We isolated a Chlamydomonas gene encoding putative γ-glutamyl kinase (GK), an enzyme that catalyzes the first step of proline biosynthesis. Using an Escherichia coli auxotroph and a purified recombinant protein, we show that Chlamydomonas GK is a functional GK. The sensitivity of this kinase to feedback inhibition by proline was lower than in that of microbial GKs previously reported. |
| 巻・号 | 112(1) |
| ページ | 75-8 |
| 公開日 | 2011-7-1 |
| DOI | 10.1016/j.jbiosc.2011.02.012 |
| PII | S1389-1723(11)00076-4 |
| PMID | 21444242 |
| MeSH | Amino Acid Sequence Chlamydomonas reinhardtii / genetics Chlamydomonas reinhardtii / metabolism* Escherichia coli / genetics Escherichia coli / metabolism Molecular Sequence Data Phosphotransferases (Carboxyl Group Acceptor) / genetics Phosphotransferases (Carboxyl Group Acceptor) / isolation & purification* Phosphotransferases (Carboxyl Group Acceptor) / metabolism Proline / metabolism Recombinant Proteins / metabolism |
| IF | 2.366 |
| 引用数 | 0 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY FOOD SCIENCE & TECHNOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | NA |