| RRC ID |
35452
|
| 著者 |
Ojima Y, Hakamada K, Nishinoue Y, Nguyen MH, Miyake J, Taya M.
|
| タイトル |
Motility behavior of rpoS-deficient Escherichia coli analyzed by individual cell tracking.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
Motility is one of the most extensively studied cellular events conducted by bacteria, including Escherichia coli. A motility agar plate assay showed that deletion of the rpoS gene enhanced the apparent motility of the E. coli BW25113 strain, which inherently had negligible motility compared to wild-type E. coli strains, such as MG1655, with no effect on cell growth. This enhancement of motility was accompanied by drastic up-regulation of genes involved in the formation and rotation of flagella. Furthermore, an individual cell motility assay showed that the population of ΔrpoS cells had bimodal motility character, and that a minority of this population exhibited a much higher motility rate. These results support a view that a minority population contributes to increasing in apparent motility of the whole population of ΔrpoS cells.
|
| 巻・号 |
114(6)
|
| ページ |
652-6
|
| 公開日 |
2012-12-1
|
| DOI |
10.1016/j.jbiosc.2012.06.014
|
| PII |
S1389-1723(12)00281-2
|
| PMID |
22846441
|
| MeSH |
Bacterial Proteins / genetics
Biological Assay
Cell Tracking
Escherichia coli / cytology*
Escherichia coli / genetics
Escherichia coli / growth & development
Escherichia coli / physiology*
Escherichia coli Proteins / genetics
Flagella / genetics
Flagella / physiology
Gene Deletion
Gene Expression Regulation, Bacterial
Movement*
Sigma Factor / deficiency*
Sigma Factor / genetics
Up-Regulation
|
| IF |
2.366
|
| 引用数 |
4
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
NA |
