RRC ID 3546
Author Shimada M, Kanematsu K, Tanaka K, Yokosawa H, Kawahara H.
Title Proteasomal ubiquitin receptor RPN-10 controls sex determination in Caenorhabditis elegans.
Journal Mol Biol Cell
Abstract The ubiquitin-binding RPN-10 protein serves as a ubiquitin receptor that delivers client proteins to the 26S proteasome. Although ubiquitin recognition is an essential step for proteasomal destruction, deletion of the rpn-10 gene in yeast does not influence viability, indicating redundancy of the substrate delivery pathway. However, their specificity and biological relevance in higher eukaryotes is still enigmatic. We report herein that knockdown of the rpn-10 gene, but not any other proteasome subunit genes, sexually transforms hermaphrodites to females by eliminating hermaphrodite spermatogenesis in Caenorhabditis elegans. The feminization phenotype induced by deletion of the rpn-10 gene was rescued by knockdown of tra-2, one of sexual fate decision genes promoting female development, and its downstream target tra-1, indicating that the TRA-2-mediated sex determination pathway is crucial for the Delta rpn-10-induced sterile phenotype. Intriguingly, we found that co-knockdown of rpn-10 and functionally related ubiquitin ligase ufd-2 overcomes the germline-musculinizing effect of fem-3(gf). Furthermore, TRA-2 proteins accumulated in rpn-10-defective worms. Our results show that the RPN-10-mediated ubiquitin pathway is indispensable for control of the TRA-2-mediated sex-determining pathway.
Volume 17(12)
Pages 5356-71
Published 2006-12-1
DOI 10.1091/mbc.e06-05-0437
PII E06-05-0437
PMID 17050737
PMC PMC1687211
MeSH Alleles Animals Base Sequence Caenorhabditis elegans / cytology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Exons / genetics Female Feminization Gene Deletion Infertility Intestinal Mucosa / metabolism Male Membrane Proteins / metabolism Molecular Sequence Data Mutant Proteins / metabolism Phenotype Proteasome Endopeptidase Complex / metabolism* Receptors, Cytoplasmic and Nuclear / genetics Receptors, Cytoplasmic and Nuclear / metabolism* Sex Determination Processes* Spermatogenesis / physiology
IF 3.791
Times Cited 34
C.elegans tm1180 tm1349 tm911