Reference - Detail
|Author||Liem PH, Mu A, Kikuta S, Ohta K, Kitajima S, Taketani S.|
|Title||A simple and highly sensitive method of measuring heme oxygenase activity.|
Heme oxygenase (HO) is a rate-limiting step of heme degradation, which catalyzes the conversion of heme into biliverdin, iron, and CO. HO has been characterized in microorganisms, insects, plants, and mammals. Previously used assays of HO activity were complicated and had low sensitivity. We found that the use of an eel bilirubin-bound fluorescent protein, UnaG, can achieve a highly sensitive and simple assay of HO activity. Using several enzyme sources including human culture cells, homogenates of plant tissues, and recombinant yeast HO, data were successfully obtained. The present method can facilitate the examination of HO in various organisms.
|MeSH||Animals Bilirubin / chemistry* Bilirubin / metabolism Eels Enzyme Activation Heme Oxygenase (Decyclizing) / metabolism* Hep G2 Cells Humans Luminescent Proteins / chemistry* Luminescent Proteins / metabolism Plant Leaves / enzymology Plant Roots / enzymology Saccharomyces cerevisiae / enzymology Tobacco / enzymology Tumor Cells, Cultured|
|WOS Category||BIOCHEMISTRY & MOLECULAR BIOLOGY|
|DNA material||pET-His UNAG (RDB13504)|