RRC ID 35467
Author Liem PH, Mu A, Kikuta S, Ohta K, Kitajima S, Taketani S.
Title A simple and highly sensitive method of measuring heme oxygenase activity.
Journal Biol. Chem.
Abstract Heme oxygenase (HO) is a rate-limiting step of heme degradation, which catalyzes the conversion of heme into biliverdin, iron, and CO. HO has been characterized in microorganisms, insects, plants, and mammals. Previously used assays of HO activity were complicated and had low sensitivity. We found that the use of an eel bilirubin-bound fluorescent protein, UnaG, can achieve a highly sensitive and simple assay of HO activity. Using several enzyme sources including human culture cells, homogenates of plant tissues, and recombinant yeast HO, data were successfully obtained. The present method can facilitate the examination of HO in various organisms.
Volume 396(11)
Pages 1265-8
Published 2015-11
DOI 10.1515/hsz-2015-0176
PII /j/bchm.just-accepted/hsz-2015-0176/hsz-2015-0176.xml
PMID 26040009
MeSH Animals Bilirubin / chemistry* Bilirubin / metabolism Eels Enzyme Activation Heme Oxygenase (Decyclizing) / metabolism* Hep G2 Cells Humans Luminescent Proteins / chemistry* Luminescent Proteins / metabolism Plant Leaves / enzymology Plant Roots / enzymology Saccharomyces cerevisiae / enzymology Tobacco / enzymology Tumor Cells, Cultured
IF 3.022
Times Cited 3
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material pET-His UNAG (RDB13504)