論文 - 詳細
| RRC ID | 35467 |
|---|---|
| 著者 | Liem PH, Mu A, Kikuta S, Ohta K, Kitajima S, Taketani S. |
| タイトル | A simple and highly sensitive method of measuring heme oxygenase activity. |
| ジャーナル | Biol Chem |
| Abstract |
Heme oxygenase (HO) is a rate-limiting step of heme degradation, which catalyzes the conversion of heme into biliverdin, iron, and CO. HO has been characterized in microorganisms, insects, plants, and mammals. Previously used assays of HO activity were complicated and had low sensitivity. We found that the use of an eel bilirubin-bound fluorescent protein, UnaG, can achieve a highly sensitive and simple assay of HO activity. Using several enzyme sources including human culture cells, homogenates of plant tissues, and recombinant yeast HO, data were successfully obtained. The present method can facilitate the examination of HO in various organisms. |
| 巻・号 | 396(11) |
| ページ | 1265-8 |
| 公開日 | 2015-11-1 |
| DOI | 10.1515/hsz-2015-0176 |
| PII | /j/bchm.just-accepted/hsz-2015-0176/hsz-2015-0176.xml |
| PMID | 26040009 |
| MeSH | Animals Bilirubin / chemistry* Bilirubin / metabolism Eels Enzyme Activation Heme Oxygenase (Decyclizing) / metabolism* Hep G2 Cells Humans Luminescent Proteins / chemistry* Luminescent Proteins / metabolism Plant Leaves / enzymology Plant Roots / enzymology Saccharomyces cerevisiae / enzymology Tobacco / enzymology Tumor Cells, Cultured |
| IF | 3.27 |
| 引用数 | 3 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 遺伝子材料 | pET-His UNAG (RDB13504) |