RRC ID 35479
著者 Kimura S, Ikeuchi Y, Kitahara K, Sakaguchi Y, Suzuki T, Suzuki T.
タイトル Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity.
ジャーナル Nucleic Acids Res.
Abstract Modifications of rRNAs are clustered in functional regions of the ribosome. In Helix 74 of Escherichia coli 23S rRNA, guanosines at positions 2069 and 2445 are modified to 7-methylguanosine(m(7)G) and N(2)-methylguanosine(m(2)G), respectively. We searched for the gene responsible for m(7)G2069 formation, and identified rlmL, which encodes the methyltransferase for m(2)G2445, as responsible for the biogenesis of m(7)G2069. In vitro methylation of rRNA revealed that rlmL encodes a fused methyltransferase responsible for forming both m(7)G2069 and m(2)G2445. We renamed the gene rlmKL. The N-terminal RlmL activity for m(2)G2445 formation was significantly enhanced by the C-terminal RlmK. Moreover, RlmKL had an unwinding activity of Helix 74, facilitating cooperative methylations of m(7)G2069 and m(2)G2445 during biogenesis of 50S subunit. In fact, we observed that RlmKL was involved in the efficient assembly of 50S subunit in a mutant strain lacking an RNA helicase deaD.
巻・号 40(9)
ページ 4071-85
公開日 2012-5
DOI 10.1093/nar/gkr1287
PII gkr1287
PMID 22210896
PMC PMC3351187
MeSH Escherichia coli / enzymology Escherichia coli / genetics Escherichia coli Proteins / chemistry Escherichia coli Proteins / metabolism* Guanosine / analogs & derivatives* Guanosine / metabolism Methylation Methyltransferases / chemistry Methyltransferases / metabolism* Models, Molecular Protein Structure, Tertiary RNA Helicases / metabolism RNA, Double-Stranded / chemistry RNA, Double-Stranded / metabolism* RNA, Ribosomal, 23S / chemistry RNA, Ribosomal, 23S / metabolism* Ribosome Subunits, Large, Bacterial / metabolism
IF 10.162
引用数 11
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) JW0931-KC OCL/R-series ME4805(OCR55)