| RRC ID |
35513
|
| 著者 |
Santos CA, Toledo MA, Trivella DB, Beloti LL, Schneider DR, Saraiva AM, Crucello A, Azzoni AR, Souza AA, Aparicio R, Souza AP.
|
| タイトル |
Functional and structural studies of the disulfide isomerase DsbC from the plant pathogen Xylella fastidiosa reveals a redox-dependent oligomeric modulation in vitro.
|
| ジャーナル |
FEBS J
|
| Abstract |
Xylella fastidiosa is a Gram-negative bacterium that grows as a biofilm inside the xylem vessels of susceptible plants and causes several economically relevant crop diseases. In the present study, we report the functional and low-resolution structural characterization of the X. fastidiosa disulfide isomerase DsbC (XfDsbC). DsbC is part of the disulfide bond reduction/isomerization pathway in the bacterial periplasm and plays an important role in oxidative protein folding. In the present study, we demonstrate the presence of XfDsbC during different stages of X. fastidiosa biofilm development. XfDsbC was not detected during X. fastidiosa planktonic growth; however, after administering a sublethal copper shock, we observed an overexpression of XfDsbC that also occurred during planktonic growth. These results suggest that X. fastidiosa can use XfDsbC in vivo under oxidative stress conditions similar to those induced by copper. In addition, using dynamic light scattering and small-angle X-ray scattering, we observed that the oligomeric state of XfDsbC in vitro may be dependent on the redox environment. Under reducing conditions, XfDsbC is present as a dimer, whereas a putative tetrameric form was observed under nonreducing conditions. Taken together, our findings demonstrate the overexpression of XfDsbC during biofilm formation and provide the first structural model of a bacterial disulfide isomerase in solution.
|
| 巻・号 |
279(20)
|
| ページ |
3828-43
|
| 公開日 |
2012-10-1
|
| DOI |
10.1111/j.1742-4658.2012.08743.x
|
| PMID |
22889056
|
| MeSH |
Amino Acid Sequence
Bacterial Proteins / chemistry*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Biofilms / drug effects
Biofilms / growth & development
Copper / pharmacology
Escherichia coli / enzymology
Escherichia coli / genetics
Escherichia coli / growth & development
Genetic Complementation Test
Models, Molecular
Molecular Sequence Data
Mutation
Oxidation-Reduction
Plant Diseases / microbiology
Protein Disulfide-Isomerases / chemistry*
Protein Disulfide-Isomerases / genetics
Protein Disulfide-Isomerases / metabolism
Protein Multimerization*
Protein Structure, Quaternary
Scattering, Small Angle
Sequence Homology, Amino Acid
X-Ray Diffraction
Xylella / enzymology*
Xylella / genetics
Xylella / physiology
|
| IF |
4.392
|
| 引用数 |
3
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
JW2861(ASKA library)
ME9062(BW25113) |
