| RRC ID |
35601
|
| Author |
Wei Y, Gao ZQ, Otsuka Y, Naka K, Yonesaki T, Zhang H, Dong YH.
|
| Title |
Structure-function studies of Escherichia coli RnlA reveal a novel toxin structure involved in bacteriophage resistance.
|
| Journal |
Mol Microbiol
|
| Abstract |
Escherichia coli RnlA-RnlB is a newly identified toxin-antitoxin (TA) system that plays a role in bacteriophage resistance. RnlA functions as a toxin with mRNA endoribonuclease activity and the cognate antitoxin RnlB inhibits RnlA toxicity in E. coli cells. Interestingly, T4 phage encodes the antitoxin Dmd, which acts against RnlA to promote its own propagation, suggesting that RnlA-Dmd represents a novel TA system. Here, we have determined the crystal structure of RnlA refined to 2.10 (Dmd-binding domain), which is an organization not previously observed among known toxin structures. Small-angle X-ray scattering (SAXS) analysis revealed that RnlA forms a dimer in solution via interactions between the DBDs from both monomers. The in vitro and in vivo functional studies showed that among the three domains, only the DBD is responsible for recognition and inhibition by Dmd and subcellular location of RnlA. In particular, the helix located at the C-terminus of DBD plays a vital role in binding Dmd. Our comprehensive studies reveal the key region responsible for RnlA toxicity and provide novel insights into its structure-function relationship.
|
| Volume |
90(5)
|
| Pages |
956-65
|
| Published |
2013-12-1
|
| DOI |
10.1111/mmi.12409
|
| PMID |
24112600
|
| MeSH |
Bacterial Toxins / chemistry*
Binding Sites
Crystallography, X-Ray
Escherichia coli K12 / genetics*
Escherichia coli K12 / metabolism*
Escherichia coli Proteins / chemistry*
Escherichia coli Proteins / metabolism
Escherichia coli Proteins / physiology*
Gene Expression Regulation, Bacterial
Models, Molecular
Protein Conformation
Protein Multimerization
Protein Structure, Secondary
Protein Structure, Tertiary
Recombinant Proteins / chemistry
Scattering, Small Angle
Sequence Homology, Amino Acid
Viral Proteins / chemistry
Viral Proteins / metabolism*
|
| IF |
3.418
|
| Times Cited |
14
|
|
WOS Category
|
MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Prokaryotes E. coli |
|
