RRC ID 3561
Author Audhya A, Desai A, Oegema K.
Title A role for Rab5 in structuring the endoplasmic reticulum.
Journal J Cell Biol
Abstract The endoplasmic reticulum (ER) is a contiguous network of interconnected membrane sheets and tubules. The ER is differentiated into distinct domains, including the peripheral ER and nuclear envelope. Inhibition of two ER proteins, Rtn4a and DP1/NogoA, was previously shown to inhibit the formation of ER tubules in vitro. We show that the formation of ER tubules in vitro also requires a Rab family GTPase. Characterization of the 29 Caenorhabditis elegans Rab GTPases reveals that depletion of RAB-5 phenocopies the defects in peripheral ER structure that result from depletion of RET-1 and YOP-1, the C. elegans homologues of Rtn4a and DP1/NogoA. Perturbation of endocytosis by other means did not affect ER structure; the role of RAB-5 in ER morphology is thus independent of its well-studied requirement for endocytosis. RAB-5 and YOP-1/RET-1 also control the kinetics of nuclear envelope disassembly, which suggests an important role for the morphology of the peripheral ER in this process.
Volume 178(1)
Pages 43-56
Published 2007-7-2
DOI 10.1083/jcb.200701139
PII jcb.200701139
PMID 17591921
PMC PMC2064419
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Cell Extracts / chemistry Embryo, Nonmammalian Endoplasmic Reticulum / metabolism* Female Green Fluorescent Proteins / metabolism Histones / genetics Histones / metabolism Models, Biological Ovum / chemistry RNA Interference RNA, Helminth Recombinant Fusion Proteins / metabolism Xenopus laevis rab5 GTP-Binding Proteins / metabolism*
IF 8.891
Times Cited 80
C.elegans tm1512