論文 - 詳細
| RRC ID | 35620 |
|---|---|
| 著者 | Shah AA, Wang C, Yoon SH, Kim JY, Choi ES, Kim SW. |
| タイトル | RecA-mediated SOS response provides a geraniol tolerance in Escherichia coli. |
| ジャーナル | J Biotechnol |
| Abstract |
Geraniol is an important industrial material and a potential candidate of advanced biofuels. One challenge of microbial geraniol production is the toxicity to hosts. However, the poor understanding on geraniol tolerance mechanism is an obstacle for developing geraniol tolerant host. This study genome-widely screened a shot-gun DNA library of Escherichia coli and found that recA is able to confer geraniol tolerance in E. coli. The recA knockout mutant was found extremely sensitive to geraniol. Based on our data, it was deciphered that recA provided tolerance through SOS response network responding to DNA damage caused by geraniol. RecA-mediated SOS response activates the homologous recombinational repair by RecB and RecN for corrective DNA maintenance. This protection mechanism suggests an effective strategy to combat geraniol toxicity in E. coli. |
| 巻・号 | 167(4) |
| ページ | 357-64 |
| 公開日 | 2013-9-20 |
| DOI | 10.1016/j.jbiotec.2013.07.023 |
| PII | S0168-1656(13)00329-5 |
| PMID | 23906844 |
| MeSH | Acyclic Monoterpenes Biofuels DNA Repair* DNA, Bacterial / genetics DNA, Bacterial / metabolism Drug Resistance, Bacterial Escherichia coli / drug effects* Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Knockout Techniques Gene Library Genome, Bacterial Microbial Viability / drug effects Rec A Recombinases / genetics Rec A Recombinases / metabolism* Recombinational DNA Repair / genetics SOS Response, Genetics* Terpenes / toxicity* |
| IF | 3.503 |
| 引用数 | 8 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW2669-KC JW2788-KC JW3784-KC JW5416-KC ME9062(BW25113) pCP20 |