RRC ID 35620
著者 Shah AA, Wang C, Yoon SH, Kim JY, Choi ES, Kim SW.
タイトル RecA-mediated SOS response provides a geraniol tolerance in Escherichia coli.
ジャーナル J Biotechnol
Abstract Geraniol is an important industrial material and a potential candidate of advanced biofuels. One challenge of microbial geraniol production is the toxicity to hosts. However, the poor understanding on geraniol tolerance mechanism is an obstacle for developing geraniol tolerant host. This study genome-widely screened a shot-gun DNA library of Escherichia coli and found that recA is able to confer geraniol tolerance in E. coli. The recA knockout mutant was found extremely sensitive to geraniol. Based on our data, it was deciphered that recA provided tolerance through SOS response network responding to DNA damage caused by geraniol. RecA-mediated SOS response activates the homologous recombinational repair by RecB and RecN for corrective DNA maintenance. This protection mechanism suggests an effective strategy to combat geraniol toxicity in E. coli.
巻・号 167(4)
ページ 357-64
公開日 2013-9-20
DOI 10.1016/j.jbiotec.2013.07.023
PII S0168-1656(13)00329-5
PMID 23906844
MeSH Acyclic Monoterpenes Biofuels DNA Repair* DNA, Bacterial / genetics DNA, Bacterial / metabolism Drug Resistance, Bacterial Escherichia coli / drug effects* Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Knockout Techniques Gene Library Genome, Bacterial Microbial Viability / drug effects Rec A Recombinases / genetics Rec A Recombinases / metabolism* Recombinational DNA Repair / genetics SOS Response, Genetics* Terpenes / toxicity*
IF 3.503
引用数 8
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
原核生物(大腸菌) JW2669-KC JW2788-KC JW3784-KC JW5416-KC ME9062(BW25113) pCP20