RRC ID 35636
著者 Carpenter CD, Cooley BJ, Needham BD, Fisher CR, Trent MS, Gordon V, Payne SM.
タイトル The Vps/VacJ ABC transporter is required for intercellular spread of Shigella flexneri.
ジャーナル Infect Immun
Abstract The Vps/VacJ ABC transporter system is proposed to function in maintaining the lipid asymmetry of the outer membrane. Mutations in vps or vacJ in Shigella flexneri resulted in increased sensitivity to lysis by the detergent sodium dodecyl sulfate (SDS), and the vpsC mutant showed minor differences in its phospholipid profile compared to the wild type. vpsC mutants were unable to form plaques in cultured epithelial cells, but this was not due to a failure to invade, to replicate intracellularly, or to polymerize actin via IcsA for movement within epithelial cells. The addition of the outer membrane phospholipase gene pldA on a multicopy plasmid in a vpsC or vacJ mutant restored its resistance to SDS, suggesting a restoration of lipid asymmetry to the outer membrane. However, the pldA plasmid did not restore the mutant's ability to form plaques in tissue culture cells. Increased PldA levels also failed to restore the mutant's phospholipid profile to that of the wild type. We propose a dual function of the Vps/VacJ ABC transporter system in S. flexneri in both the maintenance of lipid asymmetry in the outer membrane and the intercellular spread of the bacteria between adjacent epithelial cells.
巻・号 82(2)
ページ 660-9
公開日 2014-2-1
DOI 10.1128/IAI.01057-13
PII IAI.01057-13
PMID 24478081
PMC PMC3911398
MeSH ATP-Binding Cassette Transporters / genetics ATP-Binding Cassette Transporters / metabolism* Bacterial Outer Membrane Proteins / genetics Bacterial Outer Membrane Proteins / metabolism* Bacterial Proteins / genetics Bacterial Proteins / metabolism* Cell Line Epithelial Cells / microbiology Humans Membrane Proteins / genetics Membrane Proteins / metabolism* Mutation Phospholipids / analysis Shigella flexneri / chemistry Shigella flexneri / physiology*
IF 3.201
引用数 27
WOS 分野 INFECTIOUS DISEASES IMMUNOLOGY
リソース情報
原核生物(大腸菌) JW3794-KC