| RRC ID |
35691
|
| 著者 |
Suzuki S, Suzuki M, Sembon S, Fuchimoto D, Onishi A.
|
| タイトル |
Characterization of actions of octanoate on porcine preadipocytes and adipocytes differentiated in vitro.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Octanoate is used to induce adipogenic differentiation and/or lipid accumulation in preadipocytes of domestic animals. However, information on detailed actions of octanoate and the characteristics of octanoate-induced adipocytes is limited. The aim of this study was to examine these issues by comparing the outcomes of the effects of octanoate with those of rosiglitazone, which is a well-defined activator of peroxisome proliferator-activated receptor (PPAR)-γ. The adipocytes that were differentiated with 5mM of octanoate had dispersed and diversely sized lipid droplets compared to those that were differentiated with 1 μM of rosiglitazone. The gene expression levels of adiponectin, glycerol-3-phosphate dehydrogenase, perilipin 1, and perilipin 4 were much higher in the adipocytes that were differentiated with rosiglitazone than in those differentiated with octanoate, while the gene expression levels of lipoprotein lipase and perilipin 2 were decreased in rosiglitazone-differentiated adipocytes compared to octanoate-differentiated adipocytes. However, the expressions of aP2 and CD36 genes were comparably induced. Luciferase reporter assays revealed that PPAR and liver-X-receptor activities were upregulated by octanoate more effectively than by rosiglitazone. Overall, these results suggested that the action of octanoate was complicated and may be dependent on the targeted genes and cellular status.
|
| 巻・号 |
432(1)
|
| ページ |
92-8
|
| 公開日 |
2013-3-1
|
| DOI |
10.1016/j.bbrc.2013.01.080
|
| PII |
S0006-291X(13)00159-9
|
| PMID |
23376076
|
| MeSH |
Adipocytes / cytology
Adipocytes / drug effects*
Adipogenesis / drug effects*
Adipogenesis / genetics
Adiposity / drug effects*
Animals
Caprylates / pharmacology*
Cells, Cultured
Cytosol / metabolism
Dexamethasone / pharmacology
Gene Expression Regulation / drug effects
Genes, Reporter / drug effects
Insulin / pharmacology
Liver X Receptors
Orphan Nuclear Receptors / genetics
PPAR gamma / genetics
Swine / metabolism*
Triglycerides / metabolism
|
| IF |
2.985
|
| 引用数 |
4
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
CS-CDF-CG-PRE (RDB04379)
CSII-CMV-MCS-IRES2-Bsd (RDB04385)
pCAG-HIVgp (RDB04394)
pCMV-VSV-G-RSV-Rev (RDB04393). |
