論文 - 詳細
| RRC ID | 3570 |
|---|---|
| 著者 | Estes KA, Kalamegham R, Hanna-Rose W. |
| タイトル | Membrane localization of the NlpC/P60 family protein EGL-26 correlates with regulation of vulval cell morphogenesis in Caenorhabditis elegans. |
| ジャーナル | Dev Biol |
| Abstract |
Vulval morphogenesis in Caenorhabditis elegans generates a stack of toroidal cells enclosing a tubular lumen. Mutation of egl-26 is associated with malformation of vulF, the most dorsal toroid in the stack, resulting in a blocked lumen and an egg-laying defect. Here we present evidence that vulF retains the expected gene expression pattern, functions in signaling to the uterus and retains proper polarity when egl-26 is mutated, all suggesting that mutation of egl-26 specifically results in aberrant morphogenesis as opposed to abnormal fate specification. Recent computational analysis indicates that EGL-26, which was previously characterized as novel, belongs to the LRAT (lecithin retinol acyltransferase) subfamily of the NlpC/P60 superfamily of catalytic proteins. Via site-directed mutagenesis, we demonstrate a requirement of the putative catalytic residues for EGL-26 function in vivo. We also show that mutation of conserved serine 275 perturbs the apical membrane localization and the function of the EGL-26 protein. Additional mutagenesis of this residue suggests that EGL-26 attains its membrane localization via a mechanism distinct from that of LRAT. |
| 巻・号 | 308(1) |
| ページ | 196-205 |
| 公開日 | 2007-8-1 |
| DOI | 10.1016/j.ydbio.2007.05.020 |
| PII | S0012-1606(07)01044-5 |
| PMID | 17560977 |
| MeSH | Acyltransferases / genetics Acyltransferases / metabolism Animals Animals, Genetically Modified Base Sequence Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* DNA Primers / genetics DNA, Helminth / genetics Female Gene Expression Regulation, Developmental Genes, Helminth Membranes / metabolism Morphogenesis Mutagenesis, Site-Directed Mutation Signal Transduction Vulva / cytology Vulva / growth & development Vulva / metabolism |
| IF | 2.896 |
| 引用数 | 6 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm1244 |