RRC ID 3573
Author Gengyo-Ando K, Kuroyanagi H, Kobayashi T, Murate M, Fujimoto K, Okabe S, Mitani S.
Title The SM protein VPS-45 is required for RAB-5-dependent endocytic transport in Caenorhabditis elegans.
Journal EMBO Rep.
Abstract Rab5, a small guanosine triphosphatase, is known to regulate the tethering and docking reaction leading to SNARE (soluble NSF attachment protein receptors)-mediated fusion between endosomes. However, it is uncertain how the signal of the activated Rab5 protein is transduced by its downstream effectors during endosome fusion. Here, we show that the Sec1/Munc18 gene vps-45 is essential for not only viability and development but also receptor-mediated and fluid-phase endocytosis pathways in Caenorhabditis elegans. We found that VPS-45 interacts with a Rab5 effector, Rabenosyn-5 (RABS-5), and the mutants of both vps-45 and rabs-5 show similar endocytic phenotypes. In the macrophage-like cells of vps-45 and rabs-5 mutants, aberrantly small endosomes were accumulated, and the endosome fusion stimulated by the mutant RAB-5 (Q78L) is suppressed by these mutations. Our results indicate that VPS-45 is a key molecule that functions downstream from RAB-5, cooperating with RABS-5, to regulate the dynamics of the endocytic system in multicellular organisms.
Volume 8(2)
Pages 152-7
Published 2007-2
DOI 10.1038/sj.embor.7400882
PII 7400882
PMID 17235359
PMC PMC1796764
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Endocytosis / genetics Endocytosis / physiology* Microscopy, Fluorescence Munc18 Proteins / genetics Munc18 Proteins / metabolism* Mutation / genetics Signal Transduction / genetics* Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism* rab5 GTP-Binding Proteins / metabolism
IF 8.749
Times Cited 28
C.elegans tm246 tm2037 tm1560 tm2036