論文 - 詳細
| RRC ID | 3573 |
|---|---|
| 著者 | Gengyo-Ando K, Kuroyanagi H, Kobayashi T, Murate M, Fujimoto K, Okabe S, Mitani S. |
| タイトル | The SM protein VPS-45 is required for RAB-5-dependent endocytic transport in Caenorhabditis elegans. |
| ジャーナル | EMBO Rep |
| Abstract |
Rab5, a small guanosine triphosphatase, is known to regulate the tethering and docking reaction leading to SNARE (soluble NSF attachment protein receptors)-mediated fusion between endosomes. However, it is uncertain how the signal of the activated Rab5 protein is transduced by its downstream effectors during endosome fusion. Here, we show that the Sec1/Munc18 gene vps-45 is essential for not only viability and development but also receptor-mediated and fluid-phase endocytosis pathways in Caenorhabditis elegans. We found that VPS-45 interacts with a Rab5 effector, Rabenosyn-5 (RABS-5), and the mutants of both vps-45 and rabs-5 show similar endocytic phenotypes. In the macrophage-like cells of vps-45 and rabs-5 mutants, aberrantly small endosomes were accumulated, and the endosome fusion stimulated by the mutant RAB-5 (Q78L) is suppressed by these mutations. Our results indicate that VPS-45 is a key molecule that functions downstream from RAB-5, cooperating with RABS-5, to regulate the dynamics of the endocytic system in multicellular organisms. |
| 巻・号 | 8(2) |
| ページ | 152-7 |
| 公開日 | 2007-2-1 |
| DOI | 10.1038/sj.embor.7400882 |
| PII | 7400882 |
| PMID | 17235359 |
| PMC | PMC1796764 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Endocytosis / genetics Endocytosis / physiology* Microscopy, Fluorescence Munc18 Proteins / genetics Munc18 Proteins / metabolism* Mutation / genetics Signal Transduction / genetics* Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism* rab5 GTP-Binding Proteins / metabolism |
| IF | 7.497 |
| 引用数 | 37 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm246 tm2037 tm1560 tm2036 |