論文 - 詳細
| RRC ID | 35752 |
|---|---|
| 著者 | Yamamoto N, Oshima M, Tanaka C, Ogawa M, Nakajima K, Ishida K, Moriyama K, Tsuji T. |
| タイトル | Functional tooth restoration utilising split germs through re-regionalisation of the tooth-forming field. |
| ジャーナル | Sci Rep |
| Abstract |
The tooth is an ectodermal organ that arises from a tooth germ under the regulation of reciprocal epithelial-mesenchymal interactions. Tooth morphogenesis occurs in the tooth-forming field as a result of reaction-diffusion waves of specific gene expression patterns. Here, we developed a novel mechanical ligation method for splitting tooth germs to artificially regulate the molecules that control tooth morphology. The split tooth germs successfully developed into multiple correct teeth through the re-regionalisation of the tooth-forming field, which is regulated by reaction-diffusion waves in response to mechanical force. Furthermore, split teeth erupted into the oral cavity and restored physiological tooth function, including mastication, periodontal ligament function and responsiveness to noxious stimuli. Thus, this study presents a novel tooth regenerative technology based on split tooth germs and the re-regionalisation of the tooth-forming field by artificial mechanical force. |
| 巻・号 | 5 |
| ページ | 18393 |
| 公開日 | 2015-12-17 |
| DOI | 10.1038/srep18393 |
| PII | srep18393 |
| PMID | 26673152 |
| PMC | PMC4682098 |
| MeSH | Animals Biomechanical Phenomena / genetics Biomechanical Phenomena / physiology Dental Restoration, Permanent / methods* Dental Restoration, Temporary / methods* Gene Expression / genetics In Situ Hybridization Mice, Inbred C57BL Mice, Transgenic Odontogenesis / genetics Odontogenesis / physiology* Periodontal Ligament / metabolism Periodontal Ligament / physiology Periodontal Ligament / surgery Regeneration / genetics Regeneration / physiology Reverse Transcriptase Polymerase Chain Reaction Tooth / metabolism Tooth / physiology* Tooth / surgery Tooth Germ / metabolism Tooth Germ / physiology* Tooth Germ / surgery |
| IF | 3.998 |
| 引用数 | 6 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| 実験動物マウス | RBRC02707 |