RRC ID 35791
Author Ohnishi T, Shirane M, Hashimoto Y, Saita S, Nakayama KI.
Title Identification and characterization of a neuron-specific isoform of protrudin.
Journal Genes Cells
Abstract Protrudin is a membrane protein that regulates polarized vesicular transport. Now, we have identified a novel isoform of protrudin (protrudin-L) that contains an additional seven amino acids between the FFAT motif and the coiled-coil domain compared with the conventional isoform (protrudin-S) as a result of alternative splicing of a microexon (exon L). Protrudin-L mRNA was found to be mostly restricted to the central nervous system in mice, whereas protrudin-S mRNA was detected in all tissues examined. With the use of a splicing reporter minigene that produces two distinct fluorescent proteins in a manner dependent on the splicing pattern of protrudin transcripts, we found that most neurons express protrudin-L, whereas astrocytes express both protrudin isoforms and oligodendrocytes express only protrudin-S. Protrudin-L associated to a greater extent with vesicle-associated membrane protein-associated protein (VAP) than protrudin-S. Expression of protrudin-L in hippocampal neurons of protrudin-deficient mice also promoted neurite outgrowth more efficiently than protrudin-S. Our results suggest that protrudin-L is a neuron-specific protrudin isoform that promotes axonal elongation and contributes to the establishment of neuronal polarity.
Volume 19(2)
Pages 97-111
Published 2014-2-1
DOI 10.1111/gtc.12109
PMID 24251978
MeSH Alternative Splicing Animals Cell Line, Tumor Mice Mice, Inbred C57BL Molecular Sequence Data Neurons / metabolism* Organ Specificity Protein Isoforms / genetics Protein Isoforms / metabolism R-SNARE Proteins / genetics R-SNARE Proteins / metabolism* Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism*
IF 1.922
Times Cited 13
WOS Category GENETICS & HEREDITY CELL BIOLOGY
Resource
DNA material CSII-CMV-MCS (RDB04377) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394).