| RRC ID |
3582
|
| Author |
Kobayashi T, Gengyo-Ando K, Ishihara T, Katsura I, Mitani S.
|
| Title |
IFT-81 and IFT-74 are required for intraflagellar transport in C. elegans.
|
| Journal |
Genes Cells
|
| Abstract |
Intraflagellar transport (IFT) is essential machinery for biogenesis and maintenance of cilia in many eukaryotic and prokaryotic cells. A large number of polypeptides are known to be involved in IFT, but the physiological role of each component is not fully elucidated. Here, we identified a C. elegans orthologue of a Chlamydomonas reinhardtii IFT component, IFT-81, and found that its loss-of-function mutants show an unusual behavioral property and small body size. IFT-81 is expressed in sensory neurons, and localized at the base of cilia. The similar phenotypes with ift-81 mutants were also observed in several IFT mutants, suggesting these defects are caused by inability of IFT. We also demonstrated that IFT-81 interacts and co-localizes with IFT-74, which is another putative component of IFT. The ift-74 loss-of-function mutants showed phenocopies with ift-81 mutants, suggesting IFT-81 and IFT-74 play comparable functions. Moreover, ift-81 and ift-74 mutants similarly exhibited weak anomalies in cilia formation and obvious disruptions of transport in mature cilia. Thus, we conclude that IFT-81 and IFT-74 coordinately act in IFT in C. elegans sensory cilia.
|
| Volume |
12(5)
|
| Pages |
593-602
|
| Published |
2007-5-1
|
| DOI |
10.1111/j.1365-2443.2007.01076.x
|
| PII |
GTC1076
|
| PMID |
17535250
|
| MeSH |
Animals
Animals, Genetically Modified
Biological Transport, Active
Caenorhabditis elegans / genetics
Caenorhabditis elegans / physiology*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / physiology*
Carrier Proteins / genetics
Carrier Proteins / physiology*
Chemotaxis
Cilia / physiology
Flagella / physiology*
Genes, Helminth
Mutation
Neurons, Afferent / physiology
Phenotype
|
| IF |
1.655
|
| Times Cited |
20
|
|
WOS Category
|
GENETICS & HEREDITY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm2355
tm2356
tm2389
tm2394
tm2397 |
