Reference - Detail
|Author||Juo P, Harbaugh T, Garriga G, Kaplan JM.|
|Title||CDK-5 regulates the abundance of GLR-1 glutamate receptors in the ventral cord of Caenorhabditis elegans.|
|Journal||Mol. Biol. Cell|
The proline-directed kinase Cdk5 plays a role in several aspects of neuronal development. Here, we show that CDK-5 activity regulates the abundance of the glutamate receptor GLR-1 in the ventral cord of Caenorhabditis elegans and that it produces corresponding changes in GLR-1-dependent behaviors. Loss of CDK-5 activity results in decreased abundance of GLR-1 in the ventral cord, accompanied by accumulation of GLR-1 in neuronal cell bodies. Genetic analysis of cdk-5 and the clathrin adaptin unc-11 AP180 suggests that CDK-5 functions prior to endocytosis at the synapse. The scaffolding protein LIN-10/Mint-1 also regulates GLR-1 abundance in the nerve cord. CDK-5 phosphorylates LIN-10/Mint-1 in vitro and bidirectionally regulates the abundance of LIN-10/Mint-1 in the ventral cord. We propose that CDK-5 promotes the anterograde trafficking of GLR-1 and that phosphorylation of LIN-10 may play a role in this process.
|MeSH||Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans Proteins / metabolism* Cyclin-Dependent Kinase 5 / chemistry Cyclin-Dependent Kinase 5 / metabolism* Down-Regulation / genetics Endocytosis Membrane Proteins / metabolism Mutation / genetics Nervous System / enzymology* Phosphorylation Protein Structure, Tertiary Protein Subunits / metabolism R-SNARE Proteins / metabolism Receptors, AMPA / metabolism* Recombinant Fusion Proteins / metabolism|
|WOS Category||CELL BIOLOGY|