論文 - 詳細
| RRC ID | 3602 |
|---|---|
| 著者 | Maruyama R, Velarde NV, Klancer R, Gordon S, Kadandale P, Parry JM, Hang JS, Rubin J, Stewart-Michaelis A, Schweinsberg P, Grant BD, Piano F, Sugimoto A, Singson A. |
| タイトル | EGG-3 regulates cell-surface and cortex rearrangements during egg activation in Caenorhabditis elegans. |
| ジャーナル | Curr Biol |
| Abstract |
Fertilization triggers egg activation and converts the egg into a developing embryo. The events of this egg-to-embryo transition typically include the resumption of meiosis, the reorganization of the cortical actin cytoskeleton, and the remodeling of the oocyte surface. The factors that regulate sperm-dependent egg-activation events are not well understood. Caenorhabditis elegans EGG-3, a member of the protein tyrosine phosphatase-like (PTPL) family, is essential for regulating cell-surface and cortex rearrangements during egg activation in response to sperm entry. Although fertilization occurred normally in egg-3 mutants, the polarized dispersal of F-actin is altered, a chitin eggshell is not formed, and no polar bodies are produced. EGG-3 is associated with the oocyte plasma membrane in a pattern that is similar to CHS-1 and MBK-2. CHS-1 is required for eggshell deposition, whereas MBK-2 is required for the degradation of maternal proteins during the egg-to-embryo transition. The localization of CHS-1 and EGG-3 are interdependent and both genes were required for the proper localization of MBK-2 in oocytes. Therefore, EGG-3 plays a central role in egg activation by influencing polarized F-actin dynamics and the localization or activity of molecules that are directly involved in executing the egg-to-embryo transition. |
| 巻・号 | 17(18) |
| ページ | 1555-60 |
| 公開日 | 2007-9-18 |
| DOI | 10.1016/j.cub.2007.08.011 |
| PII | S0960-9822(07)01787-3 |
| PMID | 17869112 |
| MeSH | Actins / analysis Actins / metabolism Amino Acid Motifs Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cell Membrane / metabolism Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Fertilization Green Fluorescent Proteins / analysis Molecular Sequence Data Ovum / cytology Ovum / growth & development* Ovum / metabolism Protein Tyrosine Phosphatases / chemistry Protein Tyrosine Phosphatases / metabolism Protein Tyrosine Phosphatases / physiology* Protein-Tyrosine Kinases / analysis Protein-Tyrosine Kinases / metabolism |
| IF | 9.601 |
| 引用数 | 51 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm1191 |