RRC ID 36047
Author Tsuruyama T, Hiratsuka T, Aini W, Nakamura T.
Title STAT5A Modulates Chemokine Receptor CCR6 Expression and Enhances Pre-B Cell Growth in a CCL20-Dependent Manner.
Journal J. Cell. Biochem.
Abstract Signal transducer and activator of transcription 5A (STAT5A) contributes to B-cell responses to cytokines through suppressor of cytokine signaling (Socs) genes in innate immunity. However, its direct roles in B-cell responses to chemokines are poorly understood. In this study, we examined the role of STAT5A in the innate immune response. We found that STAT5A upregulated the transcription of C-C motif receptor 6 (Ccr6) to induce responses to its ligand, CCL20. STAT5A transcriptional activity proceeded through binding to the interferon-γ activation site (GAS) element in the CCR6 promoter in the genome of pre-B cells. High levels of STAT5A and CCR6 increased CCL20-dependent colony growth of pre-B cells. In human B-lymphoblastic lymphoma with inflammation, STAT5A phosphorylation was correlated with CCR6 expression (P > 0.05 compared with that in cases without inflammation). In conclusion, our data supported our hypothesis that STAT5A enhanced the response of pre-B cells to CCL20 to promote their growth.   J. Cell. Biochem. 117: 2630-2642, 2016. © 2016 Wiley Periodicals, Inc.
Volume 117(11)
Pages 2630-42
Published 2016-11
DOI 10.1002/jcb.25558
PMID 27018255
MeSH Animals Apoptosis Blotting, Western Cell Proliferation Cells, Cultured Chemokine CCL20 / genetics Chemokine CCL20 / metabolism* Cytokines / genetics Cytokines / metabolism Humans Immunoenzyme Techniques Inflammation / genetics Inflammation / metabolism Inflammation / pathology* Lymphoma, B-Cell / genetics Lymphoma, B-Cell / metabolism Lymphoma, B-Cell / pathology* Mice Phosphorylation Precursor Cells, B-Lymphoid / cytology* Precursor Cells, B-Lymphoid / metabolism RNA, Messenger / genetics Real-Time Polymerase Chain Reaction Receptors, CCR6 / genetics Receptors, CCR6 / metabolism* Reverse Transcriptase Polymerase Chain Reaction STAT5 Transcription Factor / genetics STAT5 Transcription Factor / metabolism* Signal Transduction
IF 3.448
Times Cited 1
Mice SL/Kh(RBRC00222)