RRC ID |
36047
|
著者 |
Tsuruyama T, Hiratsuka T, Aini W, Nakamura T.
|
タイトル |
STAT5A Modulates Chemokine Receptor CCR6 Expression and Enhances Pre-B Cell Growth in a CCL20-Dependent Manner.
|
ジャーナル |
J Cell Biochem
|
Abstract |
Signal transducer and activator of transcription 5A (STAT5A) contributes to B-cell responses to cytokines through suppressor of cytokine signaling (Socs) genes in innate immunity. However, its direct roles in B-cell responses to chemokines are poorly understood. In this study, we examined the role of STAT5A in the innate immune response. We found that STAT5A upregulated the transcription of C-C motif receptor 6 (Ccr6) to induce responses to its ligand, CCL20. STAT5A transcriptional activity proceeded through binding to the interferon-γ activation site (GAS) element in the CCR6 promoter in the genome of pre-B cells. High levels of STAT5A and CCR6 increased CCL20-dependent colony growth of pre-B cells. In human B-lymphoblastic lymphoma with inflammation, STAT5A phosphorylation was correlated with CCR6 expression (P > 0.05 compared with that in cases without inflammation). In conclusion, our data supported our hypothesis that STAT5A enhanced the response of pre-B cells to CCL20 to promote their growth. J. Cell. Biochem. 117: 2630-2642, 2016. © 2016 Wiley Periodicals, Inc.
|
巻・号 |
117(11)
|
ページ |
2630-42
|
公開日 |
2016-11-1
|
DOI |
10.1002/jcb.25558
|
PMID |
27018255
|
MeSH |
Animals
Apoptosis
Blotting, Western
Cell Proliferation
Cells, Cultured
Chemokine CCL20 / genetics
Chemokine CCL20 / metabolism*
Cytokines / genetics
Cytokines / metabolism
Humans
Immunoenzyme Techniques
Inflammation / genetics
Inflammation / metabolism
Inflammation / pathology*
Lymphoma, B-Cell / genetics
Lymphoma, B-Cell / metabolism
Lymphoma, B-Cell / pathology*
Mice
Phosphorylation
Precursor Cells, B-Lymphoid / cytology*
Precursor Cells, B-Lymphoid / metabolism
RNA, Messenger / genetics
Real-Time Polymerase Chain Reaction
Receptors, CCR6 / genetics
Receptors, CCR6 / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
STAT5 Transcription Factor / genetics
STAT5 Transcription Factor / metabolism*
Signal Transduction
|
IF |
4.237
|
引用数 |
7
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
実験動物マウス |
RBRC00222 |