RRC ID 3607
Author Mukhopadhyay S, Lu Y, Qin H, Lanjuin A, Shaham S, Sengupta P.
Title Distinct IFT mechanisms contribute to the generation of ciliary structural diversity in C. elegans.
Journal EMBO J.
Abstract Individual cell types can elaborate morphologically diverse cilia. Cilia are assembled via intraflagellar transport (IFT) of ciliary precursors; however, the mechanisms that generate ciliary diversity are unknown. Here, we examine IFT in the structurally distinct cilia of the ASH/ASI and the AWB chemosensory neurons in Caenorhabditis elegans, enabling us to compare IFT in specific cilia types. We show that unlike in the ASH/ASI cilia, the OSM-3 kinesin moves independently of the kinesin-II motor in the AWB cilia. Although OSM-3 is essential to extend the distal segments of the ASH/ASI cilia, it is not required to build the AWB distal segments. Mutations in the fkh-2 forkhead domain gene result in AWB-specific defects in ciliary morphology, and FKH-2 regulates kinesin-II subunit gene expression specifically in AWB. Our results suggest that cell-specific regulation of IFT contributes to the generation of ciliary diversity, and provide insights into the networks coupling the acquisition of ciliary specializations with other aspects of cell fate.
Volume 26(12)
Pages 2966-80
Published 2007-6-20
DOI 10.1038/sj.emboj.7601717
PII 7601717
PMID 17510633
PMC PMC1894762
MeSH Animals Animals, Genetically Modified Biological Transport Caenorhabditis elegans / cytology* Caenorhabditis elegans Proteins / physiology Cilia / ultrastructure* Kinesin / physiology Microscopy, Electron Mutation Olfactory Receptor Neurons / ultrastructure
IF 10.557
Times Cited 59
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
C.elegans tm324