RRC ID 3610
著者 Oosterveen T, Coudreuse DY, Yang PT, Fraser E, Bergsma J, Dale TC, Korswagen HC.
タイトル Two functionally distinct Axin-like proteins regulate canonical Wnt signaling in C. elegans.
ジャーナル Dev Biol
Abstract Axin is a central component of the canonical Wnt signaling pathway that interacts with the adenomatous polyposis coli protein APC and the kinase GSK3beta to downregulate the effector beta-catenin. In the nematode Caenorhabditis elegans, canonical Wnt signaling is negatively regulated by the highly divergent Axin ortholog PRY-1. Mutation of pry-1 leads to constitutive activation of BAR-1/beta-catenin-dependent Wnt signaling and results in a range of developmental defects. The pry-1 null phenotype is however not fully penetrant, indicating that additional factors may partially compensate for PRY-1 function. Here, we report the cloning and functional analysis of a second Axin-like protein, which we named AXL-1. We show that despite considerable sequence divergence with PRY-1 and other Axin family members, AXL-1 is a functional Axin ortholog. AXL-1 functions redundantly with PRY-1 in negatively regulating BAR-1/beta-catenin signaling in the developing vulva and the Q neuroblast lineage. In addition, AXL-1 functions independently of PRY-1 in negatively regulating canonical Wnt signaling during excretory cell development. In contrast to vertebrate Axin and the related protein Conductin, AXL-1 and PRY-1 are not functionally equivalent. We conclude that Axin function in C. elegans is divided over two different Axin orthologs that have specific functions in negatively regulating canonical Wnt signaling.
巻・号 308(2)
ページ 438-48
公開日 2007-8-15
DOI 10.1016/j.ydbio.2007.05.043
PII S0012-1606(07)01113-X
PMID 17601533
MeSH Animals Animals, Genetically Modified Axin Protein Base Sequence Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism DNA, Helminth / genetics Female Genes, Helminth Glycogen Synthase Kinase 3 / metabolism Glycogen Synthase Kinase 3 beta Repressor Proteins / genetics Repressor Proteins / metabolism* Signal Transduction Vulva / growth & development Vulva / metabolism Wnt Proteins / genetics Wnt Proteins / metabolism*
IF 2.896
引用数 17
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm1095 tm1322 tm3122