論文 - 詳細
| RRC ID | 3610 |
|---|---|
| 著者 | Oosterveen T, Coudreuse DY, Yang PT, Fraser E, Bergsma J, Dale TC, Korswagen HC. |
| タイトル | Two functionally distinct Axin-like proteins regulate canonical Wnt signaling in C. elegans. |
| ジャーナル | Dev Biol |
| Abstract |
Axin is a central component of the canonical Wnt signaling pathway that interacts with the adenomatous polyposis coli protein APC and the kinase GSK3beta to downregulate the effector beta-catenin. In the nematode Caenorhabditis elegans, canonical Wnt signaling is negatively regulated by the highly divergent Axin ortholog PRY-1. Mutation of pry-1 leads to constitutive activation of BAR-1/beta-catenin-dependent Wnt signaling and results in a range of developmental defects. The pry-1 null phenotype is however not fully penetrant, indicating that additional factors may partially compensate for PRY-1 function. Here, we report the cloning and functional analysis of a second Axin-like protein, which we named AXL-1. We show that despite considerable sequence divergence with PRY-1 and other Axin family members, AXL-1 is a functional Axin ortholog. AXL-1 functions redundantly with PRY-1 in negatively regulating BAR-1/beta-catenin signaling in the developing vulva and the Q neuroblast lineage. In addition, AXL-1 functions independently of PRY-1 in negatively regulating canonical Wnt signaling during excretory cell development. In contrast to vertebrate Axin and the related protein Conductin, AXL-1 and PRY-1 are not functionally equivalent. We conclude that Axin function in C. elegans is divided over two different Axin orthologs that have specific functions in negatively regulating canonical Wnt signaling. |
| 巻・号 | 308(2) |
| ページ | 438-48 |
| 公開日 | 2007-8-15 |
| DOI | 10.1016/j.ydbio.2007.05.043 |
| PII | S0012-1606(07)01113-X |
| PMID | 17601533 |
| MeSH | Animals Animals, Genetically Modified Axin Protein Base Sequence Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism DNA, Helminth / genetics Female Genes, Helminth Glycogen Synthase Kinase 3 / metabolism Glycogen Synthase Kinase 3 beta Repressor Proteins / genetics Repressor Proteins / metabolism* Signal Transduction Vulva / growth & development Vulva / metabolism Wnt Proteins / genetics Wnt Proteins / metabolism* |
| IF | 2.896 |
| 引用数 | 17 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm1095 tm1322 tm3122 |