論文 - 詳細
| RRC ID | 36143 |
|---|---|
| 著者 | Ito K, Shimura S, Katsuma S, Tsuda Y, Kobayashi J, Tabunoki H, Yokoyama T, Shimada T, Kadono-Okuda K. |
| タイトル | Gene expression and localization analysis of Bombyx mori bidensovirus and its putative receptor in B. mori midgut. |
| ジャーナル | J Invertebr Pathol |
| Abstract |
Bombyx mori bidensovirus (BmBDV), which causes fatal flacherie disease in the silkworm, replicates only in midgut columnar cells. The viral resistance expressed by some silkworm strains, which is characterized as non-susceptibility irrespective of the viral dose, is determined by a single gene, nsd-2. We previously identified nsd-2 by positional cloning and found that this gene encodes a putative amino acid transporter that might function as a receptor for BmBDV. In this study, we investigated the relationship between the part of the midgut expressing nsd-2 (resistance gene), +(nsd-2) (susceptibility gene) and BmBDV propagation. Quantitative RT-PCR (qRT-PCR) analysis using total RNA isolated from the anterior, middle, and posterior parts of the midgut showed that nsd-2 and +(nsd-2) were strongly expressed in the posterior part of the midgut. The expression levels of both genes were very low in the anterior and middle parts. The qRT-PCR analysis showed that the expression levels of BmBDV-derived transcripts were correlated with the levels of +(nsd-2) expression. However, BmBDV-derived transcripts were clearly detected in all parts of the midgut. These results suggest that the infectivity of BmBDV depends mainly on the expression level of +(nsd-2) in the midgut and that viral infection is supported even by very faint expression of +(nsd-2). By contrast, the expression levels of +(nsd-2) were exceedingly low or undetectable in the middle part of the midgut, indicating that BmBDV infection might occur via another mechanism, independent of +(nsd-2), in the middle part of the midgut. |
| 巻・号 | 136 |
| ページ | 50-6 |
| 公開日 | 2016-5-1 |
| DOI | 10.1016/j.jip.2016.03.005 |
| PII | S0022-2011(16)30021-0 |
| PMID | 26953258 |
| MeSH | Animals Blotting, Western Bombyx / virology* Densovirus / pathogenicity* Densovirus / physiology Digestive System / microbiology Gene Expression Profiling Genes, Insect / physiology* Genome, Viral Host-Pathogen Interactions Immunohistochemistry Real-Time Polymerase Chain Reaction Transcriptome |
| IF | 2.074 |
| 引用数 | 6 |
| WOS 分野 | ZOOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | u42 |