Reference - Detail
| RRC ID | 3616 |
|---|---|
| Author | Raharjo I, Gaudet J. |
| Title | Gland-specific expression of C. elegans hlh-6 requires the combinatorial action of three distinct promoter elements. |
| Journal | Dev Biol |
| Abstract |
The pharyngeal glands of Caenorhabditis elegans are one of five cell types in the pharynx. The transcription factor HLH-6 is required for gland development and function, and is specifically expressed in pharyngeal glands. As a first step to understanding specification of pharyngeal glands, we analyzed the promoter of hlh-6 to identify the elements required for gland-specific expression. Our experiments identified three distinct regulatory elements required for hlh-6 expression: a PHA-4-binding site and two new elements, HRL1 and HRL2 (for hlh-6 regulatory elements 1 and 2). The three elements employ a simple logic for producing cell-type-specific expression: the PHA-4 site restricts expression to the pharynx, HRL2 restricts expression in both a position and lineage-dependent manner, and HRL1 restricts expression to a subset of cell types. In isolation, these three elements have little or no enhancer activity but in combination they produce robust, gland-specific expression. These findings describe a combinatorial code for gland-specific expression and suggest that similar codes may be employed for specification of other pharyngeal cell types. |
| Volume | 302(1) |
| Pages | 295-308 |
| Published | 2007-2-1 |
| DOI | 10.1016/j.ydbio.2006.09.036 |
| PII | S0012-1606(06)01238-3 |
| PMID | 17049341 |
| MeSH | Animals Basic Helix-Loop-Helix Transcription Factors / genetics* Basic Helix-Loop-Helix Transcription Factors / metabolism Caenorhabditis / genetics Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Gene Expression Regulation* Organ Specificity Pharynx / anatomy & histology Pharynx / cytology Pharynx / metabolism Promoter Regions, Genetic* Trans-Activators / metabolism |
| IF | 2.896 |
| Times Cited | 19 |
| WOS Category | DEVELOPMENTAL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| C.elegans | tm299 |