論文 - 詳細
| RRC ID | 36178 |
|---|---|
| 著者 | Pézeron G, Millen K, Boukhatmi H, Bray S. |
| タイトル | Notch directly regulates the cell morphogenesis genes Reck, talin and trio in adult muscle progenitors. |
| ジャーナル | J Cell Sci |
| Abstract |
There is growing evidence that activation of the Notch pathway can result in consequences on cell morphogenesis and behaviour, both during embryonic development and cancer progression. In general, Notch is proposed to coordinate these processes by regulating expression of key transcription factors. However, many Notch-regulated genes identified in genome-wide studies are involved in fundamental aspects of cell behaviour, suggesting a more direct influence on cellular properties. By testing the functions of 25 such genes we confirmed that 12 are required in developing adult muscles, consistent with roles downstream of Notch. Focusing on three, Reck, rhea/talin and trio, we verify their expression in adult muscle progenitors and identify Notch-regulated enhancers in each. Full activity of these enhancers requires functional binding sites for Su(H), the DNA-binding transcription factor in the Notch pathway, validating their direct regulation. Thus, besides its well-known roles in regulating the expression of cell-fate-determining transcription factors, Notch signalling also has the potential to directly affect cell morphology and behaviour by modulating expression of genes such as Reck, rhea/talin and trio. This sheds new light on the functional outputs of Notch activation in morphogenetic processes. |
| 巻・号 | 127(Pt 21) |
| ページ | 4634-44 |
| 公開日 | 2014-11-1 |
| DOI | 10.1242/jcs.151787 |
| PII | jcs.151787 |
| PMID | 25217625 |
| PMC | PMC4215712 |
| MeSH | Animals Drosophila Drosophila Proteins / genetics Drosophila Proteins / metabolism* Guanine Nucleotide Exchange Factors / genetics Guanine Nucleotide Exchange Factors / metabolism* In Situ Hybridization Muscle Development / genetics Muscle Development / physiology Muscles / cytology Muscles / metabolism Phosphoproteins / genetics Phosphoproteins / metabolism* Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* Receptors, Notch / genetics Receptors, Notch / metabolism* Reverse Transcriptase Polymerase Chain Reaction Stem Cells / cytology* Stem Cells / metabolism* Talin / genetics Talin / metabolism* |
| IF | 4.573 |
| 引用数 | 8 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | 10701-R1 10701-R3 12489-R1 12489-R2 1283-R1 1283-R2 15288-R2 1536-R4 1822-R1 1822-R3 18314-R1 18314-R2 18430-R1 18430-R2 2008-R2 2008-R3 31352-R1 31352-R3 33275-R1 33275-R3 3937-R2 3937-R4 5392-R1 5392-R2 5730-R1 5730-R2 6831-R2 6891-R1 6891-R3 8161-R1 8161-R2 8621-R1 8621-R3 9553-R4 |