RRC ID 36197
著者 Chujo M, Yoshida S, Ota A, Murata K, Kawai S.
タイトル Acquisition of the ability to assimilate mannitol by Saccharomyces cerevisiae through dysfunction of the general corepressor Tup1-Cyc8.
ジャーナル Appl Environ Microbiol
Abstract Saccharomyces cerevisiae normally cannot assimilate mannitol, a promising brown macroalgal carbon source for bioethanol production. The molecular basis of this inability remains unknown. We found that cells capable of assimilating mannitol arose spontaneously from wild-type S. cerevisiae during prolonged culture in mannitol-containing medium. Based on microarray data, complementation analysis, and cell growth data, we demonstrated that acquisition of mannitol-assimilating ability was due to spontaneous mutations in the genes encoding Tup1 or Cyc8, which constitute a general corepressor complex that regulates many kinds of genes. We also showed that an S. cerevisiae strain carrying a mutant allele of CYC8 exhibited superior salt tolerance relative to other ethanologenic microorganisms; this characteristic would be highly beneficial for the production of bioethanol from marine biomass. Thus, we succeeded in conferring the ability to assimilate mannitol on S. cerevisiae through dysfunction of Tup1-Cyc8, facilitating production of ethanol from mannitol.
巻・号 81(1)
ページ 9-16
公開日 2015-1-1
DOI 10.1128/AEM.02906-14
PII AEM.02906-14
PMID 25304510
PMC PMC4272704
MeSH Co-Repressor Proteins / genetics Co-Repressor Proteins / metabolism* DNA, Fungal / chemistry DNA, Fungal / genetics Gene Expression Profiling Gene Expression Regulation, Fungal* Genetic Complementation Test Mannitol / metabolism* Microarray Analysis Molecular Sequence Data Mutation Nuclear Proteins / genetics Nuclear Proteins / metabolism* Repressor Proteins / genetics Repressor Proteins / metabolism* Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / growth & development Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Sequence Analysis, DNA
IF 4.016
引用数 17
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
酵母 BYP7373(pGK426)?