論文 - 詳細
| RRC ID | 36221 |
|---|---|
| 著者 | Obara K, Kihara A. |
| タイトル | Signaling events of the Rim101 pathway occur at the plasma membrane in a ubiquitination-dependent manner. |
| ジャーナル | Mol Cell Biol |
| Abstract |
In yeast, external alkalization and alteration in plasma membrane lipid asymmetry are sensed by the Rim101 pathway. It is currently under debate whether the signal elicited by external alkalization is transduced to downstream molecules at the plasma membrane or via endocytosis of the Rim21 sensor protein at the late endosome. We found that the downstream molecules, including arrestin-related protein Rim8, calpain-like protein Rim13, and scaffold protein Rim20, accumulated at the plasma membrane upon external alkalization and that the accumulation was dependent on Rim21. Snf7, an endosomal sorting complex required for transport (ESCRT) III subunit also essential for the Rim101 pathway, localized to the plasma membrane, in addition to the late endosome, under alkaline conditions. Snf7 at the plasma membrane but not at the late endosome was shown to be involved in Rim101 signaling. In addition, the Rim101 pathway was normally activated, even when endocytosis was severely impaired. Considering this information as a whole, we propose that Rim101 signaling proceeds at the plasma membrane. We also found that activity of the Rsp5 ubiquitin ligase was required for recruiting the downstream molecules to the plasma membrane, suggesting that ubiquitination mediates Rim101 signaling at the plasma membrane. |
| 巻・号 | 34(18) |
| ページ | 3525-34 |
| 公開日 | 2014-9-15 |
| DOI | 10.1128/MCB.00408-14 |
| PII | MCB.00408-14 |
| PMID | 25002535 |
| PMC | PMC4135627 |
| MeSH | Cell Cycle Proteins Cell Membrane / metabolism* Cysteine Proteases / genetics Endocytosis / genetics Endocytosis / physiology* Endosomal Sorting Complexes Required for Transport / genetics Endosomal Sorting Complexes Required for Transport / physiology* Endosomes / metabolism* Intracellular Signaling Peptides and Proteins / genetics Promoter Regions, Genetic Receptors, Cell Surface / genetics Repressor Proteins / genetics Repressor Proteins / metabolism* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Saccharomyces cerevisiae Proteins / physiology* Signal Transduction* Ubiquitin-Protein Ligase Complexes / physiology* Ubiquitination / genetics Ubiquitination / physiology |
| IF | 3.611 |
| 引用数 | 28 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | BYP6739 BYP6740 |