RRC ID 36264
Author Guven-Ozkan T, Busto GU, Schutte SS, Cervantes-Sandoval I, O'Dowd DK, Davis RL.
Title MiR-980 Is a Memory Suppressor MicroRNA that Regulates the Autism-Susceptibility Gene A2bp1.
Journal Cell Rep
Abstract MicroRNAs have been associated with many different biological functions, but little is known about their roles in conditioned behavior. We demonstrate that Drosophila miR-980 is a memory suppressor gene functioning in multiple regions of the adult brain. Memory acquisition and stability were both increased by miR-980 inhibition. Whole cell recordings and functional imaging experiments indicated that miR-980 regulates neuronal excitability. We identified the autism susceptibility gene, A2bp1, as an mRNA target for miR-980. A2bp1 levels varied inversely with miR-980 expression; memory performance was directly related to A2bp1 levels. In addition, A2bp1 knockdown reversed the memory gains produced by miR-980 inhibition, consistent with A2bp1 being a downstream target of miR-980 responsible for the memory phenotypes. Our results indicate that miR-980 represses A2bp1 expression to tune the excitable state of neurons, and the overall state of excitability translates to memory impairment or improvement.
Volume 14(7)
Pages 1698-1709
Published 2016-2-23
DOI 10.1016/j.celrep.2016.01.040
PII S2211-1247(16)30018-3
PMID 26876166
PMC PMC5103286
MeSH Animals Animals, Genetically Modified Autism Spectrum Disorder / genetics Autism Spectrum Disorder / metabolism Autism Spectrum Disorder / pathology Base Sequence Brain / growth & development Brain / metabolism* Conditioning, Classical / physiology Disease Models, Animal Drosophila Proteins / antagonists & inhibitors Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism Gene Expression Regulation, Developmental Genetic Predisposition to Disease Humans Memory / physiology* MicroRNAs / genetics* MicroRNAs / metabolism Molecular Sequence Data Olfactory Receptor Neurons / cytology Olfactory Receptor Neurons / metabolism* Protein Binding RNA, Small Interfering / genetics RNA, Small Interfering / metabolism RNA-Binding Proteins / antagonists & inhibitors RNA-Binding Proteins / genetics* RNA-Binding Proteins / metabolism Signal Transduction Transcription Factors / genetics Transcription Factors / metabolism
IF 8.109
Times Cited 22
Drosophila DGRC#113334