論文 - 詳細
| RRC ID | 36267 |
|---|---|
| 著者 | Kotani E, Yamamoto N, Kobayashi I, Uchino K, Muto S, Ijiri H, Shimabukuro J, Tamura T, Sezutsu H, Mori H. |
| タイトル | Cell proliferation by silk gut incorporating FGF-2 protein microcrystals. |
| ジャーナル | Sci Rep |
| Abstract |
Silk gut processed from the silk glands of the silkworm could be an ideal biodegradable carrier for cell growth factors. We previously demonstrated that polyhedra, microcrystals of Cypovirus 1 polyhedrin, can serve as versatile carrier proteins. Here, we report the generation of a transgenic silkworm that expresses polyhedrin together with human basic fibroblast growth factor (FGF-2) in its posterior silk glands to utilize silk gut as a proteinaceous carrier to protect and slowly release active cell growth factors. In the posterior silk glands, polyhedrin formed polyhedral microcrystals, and FGF-2 became encapsulated within the polyhedra due to a polyhedron-immobilization signal. Silk gut powder prepared from posterior silk glands containing polyhedron-encapsulated FGF-2 stimulated the phosphorylation of p44/p42 MAP kinase and induced the proliferation of serum-starved NIH3T3 cells by releasing bioactive FGF-2. Even after a one-week incubation at 25 °C, significantly higher biological activity of FGF-2 was observed for silk gut powder incorporating polyhedron-encapsulated FGF-2 relative to silk gut powder with non-encapsulated FGF-2. Our results demonstrate that posterior silk glands incorporating polyhedron-encapsulated FGF-2 are applicable to the preparation of biodegradable silk gut, which can protect and release FGF-2 that is produced in a virus- and serum-free expression system with significant application potential. |
| 巻・号 | 5 |
| ページ | 11051 |
| 公開日 | 2015-6-8 |
| DOI | 10.1038/srep11051 |
| PII | srep11051 |
| PMID | 26053044 |
| PMC | PMC4459171 |
| MeSH | Animals Animals, Genetically Modified Bombyx / genetics Bombyx / metabolism* Cell Line Cell Proliferation / drug effects* Drug Carriers / metabolism Fibroblast Growth Factor 2 / genetics Fibroblast Growth Factor 2 / metabolism Fibroblast Growth Factor 2 / pharmacology* Gene Expression / genetics Mice Mitogen-Activated Protein Kinase 1 / metabolism NIH 3T3 Cells Peptide Fragments / metabolism Phosphorylation Reoviridae / metabolism Silk / genetics Silk / metabolism* Transcription Factors Tumor Suppressor Protein p53 / metabolism |
| IF | 3.998 |
| 引用数 | 5 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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