RRC ID 36267
著者 Kotani E, Yamamoto N, Kobayashi I, Uchino K, Muto S, Ijiri H, Shimabukuro J, Tamura T, Sezutsu H, Mori H.
タイトル Cell proliferation by silk gut incorporating FGF-2 protein microcrystals.
ジャーナル Sci Rep
Abstract Silk gut processed from the silk glands of the silkworm could be an ideal biodegradable carrier for cell growth factors. We previously demonstrated that polyhedra, microcrystals of Cypovirus 1 polyhedrin, can serve as versatile carrier proteins. Here, we report the generation of a transgenic silkworm that expresses polyhedrin together with human basic fibroblast growth factor (FGF-2) in its posterior silk glands to utilize silk gut as a proteinaceous carrier to protect and slowly release active cell growth factors. In the posterior silk glands, polyhedrin formed polyhedral microcrystals, and FGF-2 became encapsulated within the polyhedra due to a polyhedron-immobilization signal. Silk gut powder prepared from posterior silk glands containing polyhedron-encapsulated FGF-2 stimulated the phosphorylation of p44/p42 MAP kinase and induced the proliferation of serum-starved NIH3T3 cells by releasing bioactive FGF-2. Even after a one-week incubation at 25 °C, significantly higher biological activity of FGF-2 was observed for silk gut powder incorporating polyhedron-encapsulated FGF-2 relative to silk gut powder with non-encapsulated FGF-2. Our results demonstrate that posterior silk glands incorporating polyhedron-encapsulated FGF-2 are applicable to the preparation of biodegradable silk gut, which can protect and release FGF-2 that is produced in a virus- and serum-free expression system with significant application potential.
巻・号 5
ページ 11051
公開日 2015-6-8
DOI 10.1038/srep11051
PII srep11051
PMID 26053044
PMC PMC4459171
MeSH Animals Animals, Genetically Modified Bombyx / genetics Bombyx / metabolism* Cell Line Cell Proliferation / drug effects* Drug Carriers / metabolism Fibroblast Growth Factor 2 / genetics Fibroblast Growth Factor 2 / metabolism Fibroblast Growth Factor 2 / pharmacology* Gene Expression / genetics Mice Mitogen-Activated Protein Kinase 1 / metabolism NIH 3T3 Cells Peptide Fragments / metabolism Phosphorylation Reoviridae / metabolism Silk / genetics Silk / metabolism* Transcription Factors Tumor Suppressor Protein p53 / metabolism
IF 3.998
引用数 5
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
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