RRC ID |
36267
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著者 |
Kotani E, Yamamoto N, Kobayashi I, Uchino K, Muto S, Ijiri H, Shimabukuro J, Tamura T, Sezutsu H, Mori H.
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タイトル |
Cell proliferation by silk gut incorporating FGF-2 protein microcrystals.
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ジャーナル |
Sci Rep
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Abstract |
Silk gut processed from the silk glands of the silkworm could be an ideal biodegradable carrier for cell growth factors. We previously demonstrated that polyhedra, microcrystals of Cypovirus 1 polyhedrin, can serve as versatile carrier proteins. Here, we report the generation of a transgenic silkworm that expresses polyhedrin together with human basic fibroblast growth factor (FGF-2) in its posterior silk glands to utilize silk gut as a proteinaceous carrier to protect and slowly release active cell growth factors. In the posterior silk glands, polyhedrin formed polyhedral microcrystals, and FGF-2 became encapsulated within the polyhedra due to a polyhedron-immobilization signal. Silk gut powder prepared from posterior silk glands containing polyhedron-encapsulated FGF-2 stimulated the phosphorylation of p44/p42 MAP kinase and induced the proliferation of serum-starved NIH3T3 cells by releasing bioactive FGF-2. Even after a one-week incubation at 25 °C, significantly higher biological activity of FGF-2 was observed for silk gut powder incorporating polyhedron-encapsulated FGF-2 relative to silk gut powder with non-encapsulated FGF-2. Our results demonstrate that posterior silk glands incorporating polyhedron-encapsulated FGF-2 are applicable to the preparation of biodegradable silk gut, which can protect and release FGF-2 that is produced in a virus- and serum-free expression system with significant application potential.
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巻・号 |
5
|
ページ |
11051
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公開日 |
2015-6-8
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DOI |
10.1038/srep11051
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PII |
srep11051
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PMID |
26053044
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PMC |
PMC4459171
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MeSH |
Animals
Animals, Genetically Modified
Bombyx / genetics
Bombyx / metabolism*
Cell Line
Cell Proliferation / drug effects*
Drug Carriers / metabolism
Fibroblast Growth Factor 2 / genetics
Fibroblast Growth Factor 2 / metabolism
Fibroblast Growth Factor 2 / pharmacology*
Gene Expression / genetics
Mice
Mitogen-Activated Protein Kinase 1 / metabolism
NIH 3T3 Cells
Peptide Fragments / metabolism
Phosphorylation
Reoviridae / metabolism
Silk / genetics
Silk / metabolism*
Transcription Factors
Tumor Suppressor Protein p53 / metabolism
|
IF |
3.998
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引用数 |
5
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WOS 分野
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BIOCHEMISTRY & MOLECULAR BIOLOGY
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リソース情報 |
カイコ |
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