論文 - 詳細
| RRC ID | 36308 |
|---|---|
| 著者 | Merkling SH, Overheul GJ, van Mierlo JT, Arends D, Gilissen C, van Rij RP. |
| タイトル | The heat shock response restricts virus infection in Drosophila. |
| ジャーナル | Sci Rep |
| Abstract |
Innate immunity is the first line of defence against pathogens and is essential for survival of the infected host. The fruit fly Drosophila melanogaster is an emerging model to study viral pathogenesis, yet antiviral defence responses remain poorly understood. Here, we describe the heat shock response, a cellular mechanism that prevents proteotoxicity, as a component of the antiviral immune response in Drosophila. Transcriptome analyses of Drosophila S2 cells and adult flies revealed strong induction of the heat shock response upon RNA virus infection. Dynamic induction patterns of heat shock pathway components were characterized in vitro and in vivo following infection with different classes of viruses. The heat shock transcription factor (Hsf), as well as active viral replication, were necessary for the induction of the response. Hsf-deficient adult flies were hypersensitive to virus infection, indicating a role of the heat shock response in antiviral defence. In accordance, transgenic activation of the heat shock response prolonged survival time after infection and enabled long-term control of virus replication to undetectable levels. Together, our results establish the heat shock response as an important constituent of innate antiviral immunity in Drosophila. |
| 巻・号 | 5 |
| ページ | 12758 |
| 公開日 | 2015-8-3 |
| DOI | 10.1038/srep12758 |
| PII | srep12758 |
| PMID | 26234525 |
| PMC | PMC4522674 |
| MeSH | Animals Animals, Genetically Modified Cells, Cultured DNA Viruses / pathogenicity DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Drosophila / immunology Drosophila / physiology Drosophila / virology* Drosophila Proteins / genetics Drosophila Proteins / metabolism Female Gene Expression Profiling Gene Expression Regulation Heat Shock Transcription Factors Heat-Shock Response / physiology* Host-Pathogen Interactions* Male RNA Interference Transcription Factors / genetics Transcription Factors / metabolism Viral Load Virus Diseases / etiology Virus Diseases / veterinary |
| IF | 3.998 |
| 引用数 | 42 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#108256 |