RRC ID 36314
Author Huang Y, Xie J, Wang T.
Title A Fluorescence-Based Genetic Screen to Study Retinal Degeneration in Drosophila.
Journal PLoS One
Abstract The Drosophila visual system has been proved to be a powerful genetic model to study eye disease such as retinal degeneration. Here, we describe a genetic method termed "Rh1::GFP ey-flp/hid" that is based on the fluorescence of GFP-tagged major rhodopsin Rh1 in the eyes of living flies and can be used to monitor the integrity of photoreceptor cells. Through combination of this method and ERG recording, we examined a collection of 667 mutants and identified 18 genes that are required for photoreceptor cell maintenance, photoresponse, and rhodopsin synthesis. Our findings demonstrate that this "Rh1::GFP ey-flp/hid" method enables high-throughput F1 genetic screens to rapidly and precisely identify mutations of retinal degeneration.
Volume 10(12)
Pages e0144925
Published 2015-12-14
DOI 10.1371/journal.pone.0144925
PII PONE-D-15-33106
Description The UCLA URCFG collections were obtained from the Kyoto Drosophila Genetic Resource Center
PMID 26659849
PMC PMC4684387
MeSH Animals Animals, Genetically Modified Drosophila Drosophila Proteins / genetics* Drosophila Proteins / metabolism Electroretinography Genetic Testing* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Microscopy, Fluorescence Mutation Photoreceptor Cells, Invertebrate / physiology Retinal Degeneration / genetics* Retinal Degeneration / pathology Retinal Degeneration / veterinary Rhodopsin / genetics Rhodopsin / metabolism Synaptic Transmission
IF 2.74
Times Cited 3