論文 - 詳細
| RRC ID | 36334 |
|---|---|
| 著者 | Hirakawa I, Miyagawa S, Katsu Y, Kagami Y, Tatarazako N, Kobayashi T, Kusano T, Mizutani T, Ogino Y, Takeuchi T, Ohta Y, Iguchi T. |
| タイトル | Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryziaslatipes) exposed to 17α-ethinylestradiol. |
| ジャーナル | Chemosphere |
| Abstract |
The occurrence of oocytes in the testis (testis-ova) of several fish species is often associated with exposure of estrogenic chemicals. However, induction mechanisms of the testis-ova remain to be elucidated. To develop marker genes for detecting testis-ova in the testis, adult male medaka were exposed to nominal concentration of 100 ng L(-1) of 17α-ethinylestradiol (EE2) for 3-5 weeks, and 800 ng estradiol benzoate (EB) for 3 weeks (experiment I), and a measured concentration of 20 ng L(-1) EE2 for 1-6 weeks (experiment II). Histological analysis was performed for the testis, and microarray analyses were performed for the testis, liver and brain. Microarray analysis in the estrogen-exposed medaka liver showed vitellogenin and choriogenin as estrogen responsive genes. Testis-ova were induced in the testis after 4 weeks of exposure to 100 ng L(-1) EE2, 3 weeks of exposure to 800 ng EB, and 6 weeks of exposure to 20 ng L(-1) EE2. Microarray analysis of estrogen-exposed testes revealed up-regulation of genes related to zona pellucida (ZP) and the oocytes marker gene, 42Sp50. Using quantitative RT-PCR we confirmed that Zpc5 gene can be used as a marker for the detection of testis-ova in male medaka. |
| 巻・号 | 87(7) |
| ページ | 668-74 |
| 公開日 | 2012-5-1 |
| DOI | 10.1016/j.chemosphere.2011.12.047 |
| PII | S0045-6535(11)01416-0 |
| PMID | 22230730 |
| MeSH | Animals Endocrine Disruptors / metabolism Endocrine Disruptors / toxicity Ethinyl Estradiol / metabolism Ethinyl Estradiol / toxicity* Fish Proteins / genetics Gene Expression Profiling Liver / metabolism Male Oocytes Ovum Testis / drug effects Testis / growth & development Testis / metabolism* Testis / pathology Vitellogenins / metabolism Water Pollutants, Chemical / metabolism Water Pollutants, Chemical / toxicity* |
| IF | 5.778 |
| 引用数 | 29 |
| WOS 分野 | ENVIRONMENTAL SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | HdrR-II1(IB178) |