論文 - 詳細
| RRC ID | 3647 |
|---|---|
| 著者 | Züllig S, Neukomm LJ, Jovanovic M, Charette SJ, Lyssenko NN, Halleck MS, Reutelingsperger CP, Schlegel RA, Hengartner MO. |
| タイトル | Aminophospholipid translocase TAT-1 promotes phosphatidylserine exposure during C. elegans apoptosis. |
| ジャーナル | Curr Biol |
| Abstract |
Phospholipids are distributed asymmetrically across the plasma-membrane bilayer of eukaryotic cells: Phosphatidylserine (PS), phosphatidylethanolamine, and phosphoinositides are predominantly restricted to the inner leaflet, whereas phophatidylcholine and sphingolipids are enriched on the outer leaflet [1, 2]. Exposure of PS on the cell surface is a conserved feature of apoptosis and plays an important role in promoting the clearance of apoptotic cells by phagocytosis [3]. However, the molecular mechanism that drives PS exposure remains mysterious. To address this issue, we studied cell-surface changes during apoptosis in the nematode C. elegans. Here, we show that PS exposure can readily be detected on apoptotic C. elegans cells. We generated a transgenic strain expressing a GFP::Annexin V reporter to screen for genes required for this process. Although none of the known engulfment genes was required, RNAi knockdown of the putative aminophospholipid transporter gene tat-1 abrogated PS exposure on apoptotic cells. tat-1(RNAi) also reduced the efficiency of cell-corpse clearance, suggesting that PS exposure acts as an "eat-me" signal in worms. We propose that tat-1 homologs might also play an important role in PS exposure in mammals. |
| 巻・号 | 17(11) |
| ページ | 994-9 |
| 公開日 | 2007-6-5 |
| DOI | 10.1016/j.cub.2007.05.024 |
| PII | S0960-9822(07)01403-0 |
| PMID | 17540571 |
| MeSH | Animals Apoptosis / physiology* Biomarkers Caenorhabditis elegans / cytology* Caenorhabditis elegans / enzymology Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / antagonists & inhibitors Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cell Membrane / metabolism* Cells, Cultured Embryonic Development / genetics Germ Cells / metabolism Green Fluorescent Proteins / analysis Organisms, Genetically Modified / metabolism Phosphatidylserines / metabolism* Phospholipid Transfer Proteins / antagonists & inhibitors Phospholipid Transfer Proteins / metabolism Phospholipid Transfer Proteins / physiology* RNA Interference |
| IF | 9.601 |
| 引用数 | 51 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm469 |