RRC ID 36524
Author Janssen KP, Rost R, Eichinger L, Schleicher M.
Title Characterization of CD36/LIMPII homologues in Dictyostelium discoideum.
Journal J. Biol. Chem.
Abstract The CD36/LIMPII family is ubiquitously expressed in higher eukaryotes and consists of integral membrane proteins that have in part been characterized as cell adhesion receptors, scavenger receptors, or fatty acid transporters. However, no physiological role has been defined so far for the members of this family that localize specifically to vesicular compartments rather than to the cell surface, namely lysosomal integral membrane protein type II (LIMPII) from mammals and LmpA from the amoeba Dictyostelium discoideum. LmpA, the first described CD36/LIMPII homologue from lower eukaryotes, has initially been identified as a suppressor of the profilin-minus phenotype. We report the discovery and initial characterization of two new CD36/LIMPII-related proteins, both of which share several features with LmpA: (i) their size is considerably larger than that of the CD36/LIMPII proteins from higher eukaryotes; (ii) they show the characteristic "hairpin" topology of this protein family; (iii) they are heavily N-glycosylated; and (iv) they localize to vesicular structures of putative endolysosomal origin. However, they show intriguing differences in their developmental regulation and exhibit different sorting signals of the di-leucine or tyrosine-type in their carboxyl-terminal tail domains. These features make them promising candidates as a paradigm for the study of the function and evolution of the as yet poorly understood CD36/LIMPII proteins.
Volume 276(42)
Pages 38899-910
Published 2001-10-19
DOI 10.1074/jbc.M103384200
PII M103384200
PMID 11489884
MeSH Amino Acid Sequence Amino Acids / chemistry Animals Base Sequence Blotting, Northern Blotting, Southern Blotting, Western CD36 Antigens / chemistry* CD36 Antigens / genetics Cloning, Molecular Dictyostelium / chemistry* Gene Expression Regulation, Developmental Glycosylation Leucine / chemistry Microscopy, Fluorescence Molecular Sequence Data Phenotype Phylogeny Protein Binding Protein Structure, Tertiary Protozoan Proteins* Receptors, Immunologic* Receptors, Lipoprotein / chemistry* Receptors, Lipoprotein / genetics Receptors, Scavenger Subcellular Fractions Time Factors
IF 4.106
Times Cited 15
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Cellular slime molds