RRC ID 3657
Author Kondo T, Inagaki S, Yasuda K, Kageyama Y.
Title Rapid construction of Drosophila RNAi transgenes using pRISE, a P-element-mediated transformation vector exploiting an in vitro recombination system.
Journal Genes Genet. Syst.
Abstract RNAi is a gene-silencing phenomenon mediated by double-stranded RNA (dsRNA) and has become a powerful tool to elucidate gene function. To accomplish rapid construction of transgenes expressing dsRNA in Drosophila, we developed a novel transformation vector, pRISE, which contains an inverted repeat of the attR1-ccdB-attR2 cassette for in vitro recombination and a pentameric GAL4 binding site for conditional expression. These features enabled us to construct RNAi transgenes without a complicated cloning scheme. In cultured cells and transgenic flies, pRISE constructs carrying dsRNA transgenes induced effective RNAi against an EGFP transgene and the endogenous white gene, respectively. These results indicate that pRISE is a convenient transformation vector for studies of multiple Drosophila genes for which functional information is lacking.
Volume 81(2)
Pages 129-34
Published 2006-4
PII JST.JSTAGE/ggs/81.129
PMID 16755136
MeSH ATP-Binding Cassette Transporters / metabolism Animals Animals, Genetically Modified Cells, Cultured DNA Transposable Elements / genetics* Drosophila / genetics* Drosophila Proteins / deficiency Drosophila Proteins / metabolism Eye Proteins / metabolism Genetic Vectors / chemical synthesis* RNA Interference RNA, Small Interfering / genetics* Recombination, Genetic Transformation, Genetic Transgenes*
IF 0.913
Times Cited 22
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Drosophila