| RRC ID |
3657
|
| Author |
Kondo T, Inagaki S, Yasuda K, Kageyama Y.
|
| Title |
Rapid construction of Drosophila RNAi transgenes using pRISE, a P-element-mediated transformation vector exploiting an in vitro recombination system.
|
| Journal |
Genes Genet Syst
|
| Abstract |
RNAi is a gene-silencing phenomenon mediated by double-stranded RNA (dsRNA) and has become a powerful tool to elucidate gene function. To accomplish rapid construction of transgenes expressing dsRNA in Drosophila, we developed a novel transformation vector, pRISE, which contains an inverted repeat of the attR1-ccdB-attR2 cassette for in vitro recombination and a pentameric GAL4 binding site for conditional expression. These features enabled us to construct RNAi transgenes without a complicated cloning scheme. In cultured cells and transgenic flies, pRISE constructs carrying dsRNA transgenes induced effective RNAi against an EGFP transgene and the endogenous white gene, respectively. These results indicate that pRISE is a convenient transformation vector for studies of multiple Drosophila genes for which functional information is lacking.
|
| Volume |
81(2)
|
| Pages |
129-34
|
| Published |
2006-4-1
|
| DOI |
10.1266/ggs.81.129
|
| PII |
JST.JSTAGE/ggs/81.129
|
| PMID |
16755136
|
| MeSH |
ATP-Binding Cassette Transporters / metabolism
Animals
Animals, Genetically Modified
Cells, Cultured
DNA Transposable Elements / genetics*
Drosophila / genetics*
Drosophila Proteins / deficiency
Drosophila Proteins / metabolism
Eye Proteins / metabolism
Genetic Vectors / chemical synthesis*
RNA Interference
RNA, Small Interfering / genetics*
Recombination, Genetic
Transformation, Genetic
Transgenes*
|
| IF |
0.917
|
| Times Cited |
22
|
|
WOS Category
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Drosophila |
|
