論文 - 詳細
| RRC ID | 3657 |
|---|---|
| 著者 | Kondo T, Inagaki S, Yasuda K, Kageyama Y. |
| タイトル | Rapid construction of Drosophila RNAi transgenes using pRISE, a P-element-mediated transformation vector exploiting an in vitro recombination system. |
| ジャーナル | Genes Genet Syst |
| Abstract |
RNAi is a gene-silencing phenomenon mediated by double-stranded RNA (dsRNA) and has become a powerful tool to elucidate gene function. To accomplish rapid construction of transgenes expressing dsRNA in Drosophila, we developed a novel transformation vector, pRISE, which contains an inverted repeat of the attR1-ccdB-attR2 cassette for in vitro recombination and a pentameric GAL4 binding site for conditional expression. These features enabled us to construct RNAi transgenes without a complicated cloning scheme. In cultured cells and transgenic flies, pRISE constructs carrying dsRNA transgenes induced effective RNAi against an EGFP transgene and the endogenous white gene, respectively. These results indicate that pRISE is a convenient transformation vector for studies of multiple Drosophila genes for which functional information is lacking. |
| 巻・号 | 81(2) |
| ページ | 129-34 |
| 公開日 | 2006-4-1 |
| DOI | 10.1266/ggs.81.129 |
| PII | JST.JSTAGE/ggs/81.129 |
| PMID | 16755136 |
| MeSH | ATP-Binding Cassette Transporters / metabolism Animals Animals, Genetically Modified Cells, Cultured DNA Transposable Elements / genetics* Drosophila / genetics* Drosophila Proteins / deficiency Drosophila Proteins / metabolism Eye Proteins / metabolism Genetic Vectors / chemical synthesis* RNA Interference RNA, Small Interfering / genetics* Recombination, Genetic Transformation, Genetic Transgenes* |
| IF | 0.917 |
| 引用数 | 22 |
| WOS 分野 | GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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