RRC ID 3662
Author Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, Chow TY, Hsing YI, Kitano H, Yamaguchi I, Matsuoka M.
Title GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin.
Journal Nature
Abstract Gibberellins (GAs) are phytohormones that are essential for many developmental processes in plants. It has been postulated that plants have both membrane-bound and soluble GA receptors; however, no GA receptors have yet been identified. Here we report the isolation and characterization of a new GA-insensitive dwarf mutant of rice, gid1. The GID1 gene encodes an unknown protein with similarity to the hormone-sensitive lipases, and we observed preferential localization of a GID1-green fluorescent protein (GFP) signal in nuclei. Recombinant glutathione S-transferase (GST)-GID1 had a high affinity only for biologically active GAs, whereas mutated GST-GID1 corresponding to three gid1 alleles had no GA-binding affinity. The dissociation constant for GA4 was estimated to be around 10(-7) M, enough to account for the GA dependency of shoot elongation. Moreover, GID1 bound to SLR1, a rice DELLA protein, in a GA-dependent manner in yeast cells. GID1 overexpression resulted in a GA-hypersensitive phenotype. Together, our results indicate that GID1 is a soluble receptor mediating GA signalling in rice.
Volume 437(7059)
Pages 693-8
Published 2005-9-29
DOI 10.1038/nature04028
PII nature04028
PMID 16193045
MeSH Amino Acid Sequence Binding, Competitive Cloning, Molecular Epistasis, Genetic Genes, Plant / genetics Gibberellins / metabolism* Gibberellins / pharmacology Molecular Sequence Data Mutation / genetics Oryza / drug effects Oryza / genetics* Oryza / metabolism* Phenotype Plant Proteins / chemistry Plant Proteins / genetics Plant Proteins / metabolism* Protein Binding* Solubility Sterol Esterase / chemistry
IF 41.577
Times Cited 520
Rice gid1-1: CM1320 gid1-8:TCM3029