論文 - 詳細
| RRC ID | 3662 |
|---|---|
| 著者 | Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, Chow TY, Hsing YI, Kitano H, Yamaguchi I, Matsuoka M. |
| タイトル | GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. |
| ジャーナル | Nature |
| Abstract |
Gibberellins (GAs) are phytohormones that are essential for many developmental processes in plants. It has been postulated that plants have both membrane-bound and soluble GA receptors; however, no GA receptors have yet been identified. Here we report the isolation and characterization of a new GA-insensitive dwarf mutant of rice, gid1. The GID1 gene encodes an unknown protein with similarity to the hormone-sensitive lipases, and we observed preferential localization of a GID1-green fluorescent protein (GFP) signal in nuclei. Recombinant glutathione S-transferase (GST)-GID1 had a high affinity only for biologically active GAs, whereas mutated GST-GID1 corresponding to three gid1 alleles had no GA-binding affinity. The dissociation constant for GA4 was estimated to be around 10(-7) M, enough to account for the GA dependency of shoot elongation. Moreover, GID1 bound to SLR1, a rice DELLA protein, in a GA-dependent manner in yeast cells. GID1 overexpression resulted in a GA-hypersensitive phenotype. Together, our results indicate that GID1 is a soluble receptor mediating GA signalling in rice. |
| 巻・号 | 437(7059) |
| ページ | 693-8 |
| 公開日 | 2005-9-29 |
| DOI | 10.1038/nature04028 |
| PII | nature04028 |
| PMID | 16193045 |
| MeSH | Amino Acid Sequence Binding, Competitive Cloning, Molecular Epistasis, Genetic Genes, Plant / genetics Gibberellins / metabolism* Gibberellins / pharmacology Molecular Sequence Data Mutation / genetics Oryza / drug effects Oryza / genetics* Oryza / metabolism* Phenotype Plant Proteins / chemistry Plant Proteins / genetics Plant Proteins / metabolism* Protein Binding* Solubility Sterol Esterase / chemistry |
| IF | 42.779 |
| 引用数 | 698 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| イネ | gid1-1: CM1320 gid1-8:TCM3029 |