Reference - Detail
|Author||Kuwayama H, Miyanaga Y, Urushihara H, Ueda M.|
|Title||A RabGAP regulates life-cycle duration via trimeric G-protein cascades in Dictyostelium discoideum.|
BACKGROUND:The life-cycle of cellular slime molds comprises chronobiologically regulated processes. During the growth phase, the amoeboid cells proliferate at a definite rate. Upon starvation, they synthesize cAMP as both first and second messengers in signalling pathways and form aggregates, migrating slugs, and fruiting bodies, consisting of spores and stalk cells, within 24 h. In Dictyostelium discoideum, because most growth-specific events cease during development, proliferative and heterochronic mutations are not considered to be interrelated and no genetic factor governing the entire life-cycle duration has ever been identified.
METHODOLOGY/PRINCIPAL FINDINGS:Using yeast 2-hybrid library screening, we isolated a Dictyostelium discoideum RabGAP, Dd Rbg-3, as a candidate molecule by which the Dictyostelium Gα2 subunit directs its effects. Rab GTPase-activating protein, RabGAP, acts as a negative regulator of Rab small GTPases, which orchestrate the intracellular membrane trafficking involved in cell proliferation. Deletion mutants of Dd rbg-3 exhibited an increased growth rate and a shortened developmental period, while an overexpression mutant demonstrated the opposite effects. We also show that Dd Rbg-3 interacts with 2 Gα subunits in an activity-dependent manner in vitro. Furthermore, both human and Caenorhabditis elegans rbg-3 homologs complemented the Dd rbg-3-deletion phenotype in D. discoideum, indicating that similar pathways may be generally conserved in multicellular organisms.
CONCLUSIONS/SIGNIFICANCE:Our findings suggest that Dd Rbg-3 acts as a key element regulating the duration of D. discoideum life-span potentially via trimeric G-protein cascades.
|MeSH||Amino Acid Sequence Animals Caenorhabditis elegans Cell Proliferation Dictyostelium / genetics* Dictyostelium / metabolism GTPase-Activating Proteins / genetics* GTPase-Activating Proteins / metabolism Gene Deletion Gene Expression Regulation Genetic Complementation Test Humans Life Cycle Stages / genetics* Microtubule-Associated Proteins / genetics* Microtubule-Associated Proteins / metabolism Molecular Sequence Data Protein Binding Protein Multimerization Protozoan Proteins / genetics* Protozoan Proteins / metabolism Sequence Alignment Signal Transduction Two-Hybrid System Techniques|
|WOS Category||CELL BIOLOGY|
|Cellular slime molds|