RRC ID |
3662
|
著者 |
Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, Chow TY, Hsing YI, Kitano H, Yamaguchi I, Matsuoka M.
|
タイトル |
GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin.
|
ジャーナル |
Nature
|
Abstract |
Gibberellins (GAs) are phytohormones that are essential for many developmental processes in plants. It has been postulated that plants have both membrane-bound and soluble GA receptors; however, no GA receptors have yet been identified. Here we report the isolation and characterization of a new GA-insensitive dwarf mutant of rice, gid1. The GID1 gene encodes an unknown protein with similarity to the hormone-sensitive lipases, and we observed preferential localization of a GID1-green fluorescent protein (GFP) signal in nuclei. Recombinant glutathione S-transferase (GST)-GID1 had a high affinity only for biologically active GAs, whereas mutated GST-GID1 corresponding to three gid1 alleles had no GA-binding affinity. The dissociation constant for GA4 was estimated to be around 10(-7) M, enough to account for the GA dependency of shoot elongation. Moreover, GID1 bound to SLR1, a rice DELLA protein, in a GA-dependent manner in yeast cells. GID1 overexpression resulted in a GA-hypersensitive phenotype. Together, our results indicate that GID1 is a soluble receptor mediating GA signalling in rice.
|
巻・号 |
437(7059)
|
ページ |
693-8
|
公開日 |
2005-9-29
|
DOI |
10.1038/nature04028
|
PII |
nature04028
|
PMID |
16193045
|
MeSH |
Amino Acid Sequence
Binding, Competitive
Cloning, Molecular
Epistasis, Genetic
Genes, Plant / genetics
Gibberellins / metabolism*
Gibberellins / pharmacology
Molecular Sequence Data
Mutation / genetics
Oryza / drug effects
Oryza / genetics*
Oryza / metabolism*
Phenotype
Plant Proteins / chemistry
Plant Proteins / genetics
Plant Proteins / metabolism*
Protein Binding*
Solubility
Sterol Esterase / chemistry
|
IF |
42.779
|
引用数 |
698
|
WOS 分野
|
PLANT SCIENCES
|
リソース情報 |
イネ |
gid1-1: CM1320
gid1-8:TCM3029 |