RRC ID 36720
Author Kawaharada Y, Kelly S, Nielsen MW, Hjuler CT, Gysel K, Muszyński A, Carlson RW, Thygesen MB, Sandal N, Asmussen MH, Vinther M, Andersen SU, Krusell L, Thirup S, Jensen KJ, Ronson CW, Blaise M, Radutoiu S, Stougaard J.
Title Receptor-mediated exopolysaccharide perception controls bacterial infection.
Journal Nature
Abstract Surface polysaccharides are important for bacterial interactions with multicellular organisms, and some are virulence factors in pathogens. In the legume-rhizobium symbiosis, bacterial exopolysaccharides (EPS) are essential for the development of infected root nodules. We have identified a gene in Lotus japonicus, Epr3, encoding a receptor-like kinase that controls this infection. We show that epr3 mutants are defective in perception of purified EPS, and that EPR3 binds EPS directly and distinguishes compatible and incompatible EPS in bacterial competition studies. Expression of Epr3 in epidermal cells within the susceptible root zone shows that the protein is involved in bacterial entry, while rhizobial and plant mutant studies suggest that Epr3 regulates bacterial passage through the plant's epidermal cell layer. Finally, we show that Epr3 expression is inducible and dependent on host perception of bacterial nodulation (Nod) factors. Plant-bacterial compatibility and bacterial access to legume roots is thus regulated by a two-stage mechanism involving sequential receptor-mediated recognition of Nod factor and EPS signals.
Volume 523(7560)
Pages 308-12
Published 2015-7-16
DOI 10.1038/nature14611
PII nature14611
PMID 26153863
MeSH Amino Acid Sequence Carbohydrate Sequence Lipopolysaccharides / chemistry Lipopolysaccharides / metabolism* Loteae / genetics Loteae / metabolism* Loteae / microbiology* Molecular Sequence Data Mutation / genetics Phenotype Plant Epidermis / metabolism Plant Epidermis / microbiology Plant Proteins / chemistry Plant Proteins / genetics Plant Proteins / metabolism* Plant Root Nodulation Protein Kinases / chemistry Protein Kinases / genetics Protein Kinases / metabolism Protein Structure, Tertiary Receptors, Cell Surface / chemistry Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism* Rhizobium / metabolism* Root Nodules, Plant / metabolism Root Nodules, Plant / microbiology Signal Transduction Species Specificity Suppression, Genetic / genetics Symbiosis*
IF 41.577
Times Cited 46
WOS Category MULTIDISCIPLINARY SCIENCES
Resource
Lotus / Glycine MG-113