RRC ID 36745
Author Kitamura S, Oono Y, Narumi I.
Title Arabidopsis pab1, a mutant with reduced anthocyanins in immature seeds from banyuls, harbors a mutation in the MATE transporter FFT.
Journal Plant Mol Biol
Abstract Forward genetics approaches have helped elucidate the anthocyanin biosynthetic pathway in plants. Here, we used the Arabidopsis banyuls (ban) mutant, which accumulates anthocyanins, instead of colorless proanthocyanidin precursors, in immature seeds. In contrast to standard screens for mutants lacking anthocyanins in leaves/stems, we mutagenized ban plants and screened for mutants showing differences in pigmentation of immature seeds. The pale banyuls1 (pab1) mutation caused reduced anthocyanin pigmentation in immature seeds compared with ban. Immature pab1 ban seeds contained less anthocyanins and flavonols than ban, but showed normal expression of anthocyanin biosynthetic genes. In contrast to pab1, introduction of a flavonol-less mutation into ban did not produce paler immature seeds. Map-based cloning showed that two independent pab1 alleles disrupted the MATE-type transporter gene FFT/DTX35. Complementation of pab1 with FFT confirmed that mutation in FFT causes the pab1 phenotype. During development, FFT promoter activity was detected in the seed-coat layers that accumulate flavonoids. Anthocyanins accumulate in the vacuole and FFT fused to GFP mainly localized in the vacuolar membrane. Heterologous expression of grapevine MATE-type anthocyanin transporter gene partially complemented the pab1 phenotype. These results suggest that FFT acts at the vacuolar membrane in anthocyanin accumulation in the Arabidopsis seed coat, and that our screening strategy can reveal anthocyanin-related genes that have not been found by standard screening.
Volume 90(1-2)
Pages 7-18
Published 2016-1-1
DOI 10.1007/s11103-015-0389-8
PII 10.1007/s11103-015-0389-8
PMID 26608698
MeSH Alleles Anthocyanins / metabolism* Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Biosynthetic Pathways Flavonoids / metabolism Gene Expression Genes, Reporter Membrane Transport Proteins / genetics* Membrane Transport Proteins / metabolism Mutation Phenotype Pigmentation Plant Leaves / genetics Plant Leaves / metabolism Plant Stems / genetics Plant Stems / metabolism Seeds / genetics Seeds / metabolism Vacuoles / metabolism
IF 3.302
Times Cited 11
Arabidopsis / Cultured plant cells, genes pst16145