| RRC ID |
36760
|
| Author |
Li C, Shi C, Kim J, Chen Y, Ni S, Jiang L, Zheng C, Li D, Hou J, Taichman RS, Sun H.
|
| Title |
Erythropoietin promotes bone formation through EphrinB2/EphB4 signaling.
|
| Journal |
J Dent Res
|
| Abstract |
Recent studies have demonstrated that erythropoietin (EPO) has extensive nonhematopoietic biological functions. However, little is known about how EPO regulates bone formation, although several studies suggested that EPO can affect bone homeostasis. In this study, we investigated the effects of EPO on the communication between osteoclasts and osteoblasts through the ephrinB2/EphB4 signaling pathway. We found that EPO slightly promotes osteoblastic differentiation with the increased expression of EphB4 in ST2 cells. However, EPO increased the expression of Nfatc1 and ephrinB2 but decreased the expression of Mmp9 in RAW264.7 cells, resulting in an increase of ephrinB2-expressing osteoclasts and a decrease in resorption activity. The stimulation of ephrinB2/EphB4 signaling via ephrinB2-Fc significantly promoted EPO-mediated osteoblastic differentiation in ST2 cells. EphB4 knockdown through EphB4 shRNA inhibited EPO-mediated osteoblastic phenotypes. Furthermore, in vivo assays clearly demonstrated that EPO efficiently induces new bone formation in the alveolar bone regeneration model. Taken together, these results suggest that ephrinB2/EphB4 signaling may play an important role in EPO-mediated bone formation.
|
| Volume |
94(3)
|
| Pages |
455-63
|
| Published |
2015-3-1
|
| DOI |
10.1177/0022034514566431
|
| PII |
0022034514566431
|
| PMID |
25586589
|
| PMC |
PMC4336159
|
| MeSH |
Alveolar Process / drug effects
Animals
Bone Regeneration / drug effects
Cell Culture Techniques
Cell Differentiation / drug effects
Cell Line
Ephrin-B2 / drug effects*
Erythropoietin / pharmacology*
Erythropoietin / therapeutic use
Gene Knockdown Techniques
Gene Silencing
Incisor / surgery
Macrophages / drug effects
Male
Matrix Metalloproteinase 9 / drug effects
Mesenchymal Stem Cells / drug effects
Mice
NFATC Transcription Factors / drug effects
Osteoblasts / drug effects
Osteoclasts / drug effects
Osteogenesis / drug effects*
RNA, Small Interfering / administration & dosage
Random Allocation
Rats
Rats, Wistar
Receptor, EphB4 / drug effects*
Signal Transduction / drug effects
Tooth Extraction
Tooth Socket / drug effects
|
| IF |
4.914
|
| Times Cited |
38
|
|
WOS Category
|
DENTISTRY, ORAL SURGERY & MEDICINE
|
| Resource |
| Human and Animal Cells |
ST2(RCB0224) |
