RRC ID 36765
著者 Lai YT, Sato M, Ohta S, Akamatsu K, Nakao S, Sakai Y, Ito T.
タイトル Preparation of uniform-sized hemoglobin-albumin microspheres as oxygen carriers by Shirasu porous glass membrane emulsification technique.
ジャーナル Colloids Surf B Biointerfaces
Abstract We have developed a new type of artificial oxygen carrier composed of bovine hemoglobin (bHb) and bovine serum albumin (BSA) prepared by Shirasu porous glass (SPG) membrane emulsification technique. The resultant emulsion droplets containing 10 wt% bHb and 5-20 wt% BSA were subsequently cross-linked by glutaraldehyde to form the microspheres. Due to the uniform pore structure of SPG membranes, the average diameters of bHb10-BSAm microspheres were successfully controlled at around 5 μm with a coefficient of variation of around 10%. In addition, the biocompatibility of the carriers depended on their oxyhemoglobin percentage regardless of their same size. Finally, the P50 values of these microspheres ranged from 8.08 to 11.60 mmHg, which showed a high oxygen affinity and an oxygen delivery function.
巻・号 127
ページ 1-7
公開日 2015-3-1
DOI 10.1016/j.colsurfb.2015.01.018
PII S0927-7765(15)00025-9
PMID 25635614
MeSH Animals Cattle Cell Survival Drug Compounding / methods* Emulsions / chemistry* Glass / chemistry* Hemoglobins / chemistry* Hep G2 Cells Human Umbilical Vein Endothelial Cells Humans Membranes, Artificial Microscopy, Electron, Scanning Microspheres* Optical Imaging Oxygen / chemistry* Particle Size* Porosity Protein Stability Serum Albumin, Bovine / chemistry* Shear Strength Spectrophotometry, Ultraviolet Viscosity
IF 4.389
引用数 18
WOS 分野 BIOPHYSICS MATERIALS SCIENCE, BIOMATERIALS CHEMISTRY, PHYSICAL
リソース情報
ヒト・動物細胞 Hep G2(RCB1648) RAW 264(RCB0535)